Difference between revisions of "Team:IIT Kharagpur/Notebook"
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Revision as of 07:19, 14 July 2015
Notebook Page
(Log of day-to-day work done)
WEEK-1( July 6 - July 10 )
6th July 2015
- LB media prepared (500 ml), autoclaved and stored at 4 degrees.
- 250 ml of LB agar prepared and poured into the plates (chloramphenicol antibiotic used- concentration 1 µl/ml).
7th July 2015
- DNA from wells dissolved into 10 µl distilled water. DNA kit plate instructions
- Transformed 4µl of DNA into 100µl of E.Coli DH5α. transformation procedure
- 100µl of transformed cells were plated on chloramphenicol resistant plates.and left overnight to grow.
8th July 2015
- Colonies for luxPR promoter (BBa_K546003) constructs were observed. A colony was picked and inoculated in 3 ml culture for glycerol stock preparation.
- No colony observed for luxR protein (BBa_R6002) construct.
9th July 2015
- Transformation repeated for luxR protein construct. transformation procedure
- Culture prepared for plasmid isolation for luxPR promoter. Colony was picked and inoculated in 10 ml culture.
10th July 2015
- No colony observed for luxR protein construct.
- Plasmid isolation done for luxPR promoter.
- Gel electrophoresis done for luxPR promoter.
- Lane 7 : 1kb marker
Lane 8 : luxPR promoter