Difference between revisions of "Team:UNITN-Trento"

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<h2> Welcome to iGEM 2015! </h2>
 
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
 
  
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<h4>Before you start: </h4>
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<p> Please read the following pages:</p>
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<ul>
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<li>  <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<h4> Styling your wiki </h4>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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font-family: 'BebasNeueRegular',Arial,Helvetica,sans-serif;
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h4> Editing your wiki </h4>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:UNITN-Trento&action=edit"> Click here to edit this page! </a></p>
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<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>
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<h4>Templates </h4>
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<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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<span class="title"> UNITN-Trento
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>  
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iGEM 2015 </span>
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<br /><span style="font-style:italic; color:#555; font-size:1.3em;"> Electron-production with engineered E.coli and proteorhodopsin</span>
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<div style="background-image:url('https://static.igem.org/mediawiki/2015/5/5a/Unitn_pics_team_1.jpg'); width:72%; border-radius:120px 10px / 10px 120px; height:120px;  margin:auto; margin-top:20px; background-size:100%; background-position:50% 10%"></div>
  
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<h4>Tips</h4>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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<li>Have lots of fun! </li>
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<p>The aim of our project is to develop a pseudoautotrophic <i>E. coli</i>, enabling it to live in the <span class="highlight">microbial fuel cell</span> anaerobic condition. Our idea is to engineer <span class="highlight"><i>E. coli</i></span> with <span class="highlight"><i>proteorhodopsin</i></span>, a light-powered proton pump identified in bacteria that live in deep waters. This protein is activated by light and needs retinal molecules to pump protons outward following excitation. Thus we want to engineer our bacteria with <i>blh</i>, that encodes for the enzyme that cleaves beta-carotene and produces retinal.</p>
  
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<h4>Inspiration</h4>
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<p>To increase electron production we plan to overexpress <i>pncB</i> that represents the limiting step in the NAD+ synthesis pathway. The more NAD+ is produced, the more electrons are transported by NADH. In short: light activates proteorhodopsin, increases bacterial viability, and drives to a more efficient electron production.</p>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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<h4> Uploading pictures and files </h4>
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<p>Our bacterial device will give rise to a <span class="highlight">self-sustainable system</span>, powered by sunlight during the day and by a light bulb at night. Daytime-electricity can be either used or stored to be employed as a power source for the lightbulb at night.</p>
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<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
 
  
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Revision as of 16:11, 14 July 2015

UNITN-Trento iGEM 2015
Electron-production with engineered E.coli and proteorhodopsin

The aim of our project is to develop a pseudoautotrophic E. coli, enabling it to live in the microbial fuel cell anaerobic condition. Our idea is to engineer E. coli with proteorhodopsin, a light-powered proton pump identified in bacteria that live in deep waters. This protein is activated by light and needs retinal molecules to pump protons outward following excitation. Thus we want to engineer our bacteria with blh, that encodes for the enzyme that cleaves beta-carotene and produces retinal.

To increase electron production we plan to overexpress pncB that represents the limiting step in the NAD+ synthesis pathway. The more NAD+ is produced, the more electrons are transported by NADH. In short: light activates proteorhodopsin, increases bacterial viability, and drives to a more efficient electron production.

Our bacterial device will give rise to a self-sustainable system, powered by sunlight during the day and by a light bulb at night. Daytime-electricity can be either used or stored to be employed as a power source for the lightbulb at night.