Difference between revisions of "Team:technion"

(Created page with "<html> <main> <img src="https://static.igem.org/mediawiki/2015/a/a2/Bold.jpg" alt="Bold_img" style="width:950px;height:900px;" align="right"> <a target="_blank" title="follow me o...")
 
Line 1: Line 1:
 
<html>
 
<html>
−
<main>
+
<head>
−
<img src="https://static.igem.org/mediawiki/2015/a/a2/Bold.jpg" alt="Bold_img" style="width:950px;height:900px;" align="right">
+
<meta charset="utf-8"/>
−
<a target="_blank" title="follow me on facebook" href="http://www.facebook.com/technioniGEM2015?fref=ts"><img alt="follow me on facebook" src="https://c866088.ssl.cf3.rackcdn.com/assets/facebook40x40.png" border=0 align="middle"></a>
+
−
</main>
+
<style type="text/css">
 +
 +
.abstract {
 +
font-size: 130%;
 +
}
 +
 +
.main_menu, footer {
 +
width: 650px;
 +
margin: auto;
 +
clear: both;
 +
}
 +
 +
#igem_login {
 +
float: right;
 +
color: white;
 +
background:#7f7f7f;
 +
background:rgba(0,0,0,0.2);
 +
}
 +
 +
#igem_login:hover {
 +
color: blue;
 +
}
 +
 +
#team_logo {
 +
width: 400px;
 +
height: auto;
 +
display: block;
 +
margin: auto;
 +
}
 +
 +
.facebook_link {
 +
margin-top: 5px;
 +
}
 +
 +
body {
 +
font-family: sans-serif;
 +
background-image: url("https://static.igem.org/mediawiki/2015/1/1b/Team_Technion_Background_Hair.jpg");
 +
}
 +
 +
.content {
 +
width: 700px;
 +
margin: auto;
 +
background:#7f7f7f;
 +
background:rgba(255,255,255,0.5);
 +
}
 +
 +
.abstract {
 +
position: relative;
 +
text-align: left;
 +
z-index: 1;
 +
margin-left: 10px;
 +
}
 +
 +
.abstract_title {
 +
position: relative;
 +
text-align: center;
 +
z-index: 1;
 +
}
 +
 +
 +
 +
/*************************footer****************/
 +
footer .team_name  {
 +
text-align: center;
 +
color: white;
 +
}
 +
 +
footer .connect_links {
 +
width: 130px;
 +
margin: auto;
 +
}
 +
 +
footer {
 +
background:#7f7f7f;
 +
background:rgba(0,0,0,0.5);
 +
width: 100%;
 +
}
 +
 +
footer h3 {
 +
margin-top: 0px;
 +
margin-bottom: 0px;
 +
}
 +
 +
.connect_links {
 +
display: flex;
 +
}
 +
/*************************footer-end*************/
 +
 +
 +
 +
 +
 +
/************************************************************************************/
 +
/***********************************Buttons******************************************/
 +
/************************************************************************************/
 +
 +
/*************************one****************/
 +
.button_main1 {
 +
background: #f33;
 +
color: #fff;
 +
border: 0;
 +
padding: 1em 2em;
 +
border-radius: 6px;
 +
cursor: pointer;
 +
border-bottom: 3px solid #e60000;
 +
outline: none;
 +
position: relative;
 +
overflow: hidden;
 +
}
 +
.button_main1:hover {
 +
border-bottom-width: 2px;
 +
margin-top: 0px;
 +
}
 +
.button_main1:active,
 +
.button_main1:focus {
 +
border-bottom-width: 1px;
 +
margin-top: 2px;
 +
-webkit-animation: shakeBtn 20ms infinite;
 +
animation: shakeBtn 20ms infinite;
 +
}
 +
.button_main1 .btn-text {
 +
position: relative;
 +
z-index: 1;
 +
}
 +
.button_main1 .btn-bg {
 +
position: absolute;
 +
background: -webkit-linear-gradient(top, #f99, #ff1a1a);
 +
background: linear-gradient(to bottom, #f99, #ff1a1a);
 +
top: -100%;
 +
left: 0;
 +
width: 100%;
 +
height: 200%;
 +
-webkit-transition: all 600ms ease;
 +
transition: all 600ms ease;
 +
}
 +
.button_main1:hover .btn-bg {
 +
top: 0;
 +
}
 +
/*************************one-end*************/
 +
/*************************two****************/
 +
.button_main2 {
 +
background: #f33;
 +
color: #fff;
 +
border: 0;
 +
padding: 1em 2em;
 +
border-radius: 6px;
 +
cursor: pointer;
 +
border-bottom: 3px solid #e60000;
 +
outline: none;
 +
position: relative;
 +
overflow: hidden;
 +
}
 +
.button_main2:hover {
 +
border-bottom-width: 2px;
 +
margin-top: 0px;
 +
}
 +
.button_main2:active,
 +
.button_main2:focus {
 +
border-bottom-width: 1px;
 +
margin-top: 2px;
 +
-webkit-animation: shakeBtn 20ms infinite;
 +
animation: shakeBtn 20ms infinite;
 +
}
 +
.button_main2 .btn-text {
 +
position: relative;
 +
z-index: 1;
 +
}
 +
.button_main2 .btn-bg {
 +
position: absolute;
 +
background: -webkit-linear-gradient(top, #f99, #ff1a1a);
 +
background: linear-gradient(to bottom, #f99, #ff1a1a);
 +
top: -100%;
 +
left: 0;
 +
width: 100%;
 +
height: 200%;
 +
-webkit-transition: all 600ms ease;
 +
transition: all 600ms ease;
 +
}
 +
.button_main2:hover .btn-bg {
 +
top: 0;
 +
}
 +
/*************************two-end*************/
 +
/*************************three****************/
 +
.button_main3 {
 +
background: #f33;
 +
color: #fff;
 +
border: 0;
 +
padding: 1em 2em;
 +
border-radius: 6px;
 +
cursor: pointer;
 +
border-bottom: 3px solid #e60000;
 +
outline: none;
 +
position: relative;
 +
overflow: hidden;
 +
}
 +
.button_main3:hover {
 +
border-bottom-width: 2px;
 +
margin-top: 0px;
 +
}
 +
.button_main3:active,
 +
.button_main3:focus {
 +
border-bottom-width: 1px;
 +
margin-top: 2px;
 +
-webkit-animation: shakeBtn 20ms infinite;
 +
animation: shakeBtn 20ms infinite;
 +
}
 +
.button_main3 .btn-text {
 +
position: relative;
 +
z-index: 1;
 +
}
 +
.button_main3 .btn-bg {
 +
position: absolute;
 +
background: -webkit-linear-gradient(top, #f99, #ff1a1a);
 +
background: linear-gradient(to bottom, #f99, #ff1a1a);
 +
top: -100%;
 +
left: 0;
 +
width: 100%;
 +
height: 200%;
 +
-webkit-transition: all 600ms ease;
 +
transition: all 600ms ease;
 +
}
 +
.button_main3:hover .btn-bg {
 +
top: 0;
 +
}
 +
/*************************three-end*************/
 +
/*************************four****************/
 +
.button_main4 {
 +
background: #f33;
 +
color: #fff;
 +
border: 0;
 +
padding: 1em 2em;
 +
border-radius: 6px;
 +
cursor: pointer;
 +
border-bottom: 3px solid #e60000;
 +
outline: none;
 +
position: relative;
 +
overflow: hidden;
 +
}
 +
.button_main4:hover {
 +
border-bottom-width: 2px;
 +
margin-top: 0px;
 +
}
 +
.button_main4:active,
 +
.button_main4:focus {
 +
border-bottom-width: 1px;
 +
margin-top: 2px;
 +
-webkit-animation: shakeBtn 20ms infinite;
 +
animation: shakeBtn 20ms infinite;
 +
}
 +
.button_main4 .btn-text {
 +
position: relative;
 +
z-index: 1;
 +
}
 +
.button_main4 .btn-bg {
 +
position: absolute;
 +
background: -webkit-linear-gradient(top, #f99, #ff1a1a);
 +
background: linear-gradient(to bottom, #f99, #ff1a1a);
 +
top: -100%;
 +
left: 0;
 +
width: 100%;
 +
height: 200%;
 +
-webkit-transition: all 600ms ease;
 +
transition: all 600ms ease;
 +
}
 +
.button_main4:hover .btn-bg {
 +
top: 0;
 +
}
 +
/*************************four-end*************/
 +
/*************************five****************/
 +
.button_main5 {
 +
background: #f33;
 +
color: #fff;
 +
border: 0;
 +
padding: 1em 2em;
 +
border-radius: 6px;
 +
cursor: pointer;
 +
border-bottom: 3px solid #e60000;
 +
outline: none;
 +
position: relative;
 +
overflow: hidden;
 +
}
 +
.button_main5:hover {
 +
border-bottom-width: 2px;
 +
margin-top: 0px;
 +
}
 +
.button_main5:active,
 +
.button_main5:focus {
 +
border-bottom-width: 1px;
 +
margin-top: 2px;
 +
-webkit-animation: shakeBtn 20ms infinite;
 +
animation: shakeBtn 20ms infinite;
 +
}
 +
.button_main5 .btn-text {
 +
position: relative;
 +
z-index: 1;
 +
}
 +
.button_main5 .btn-bg {
 +
position: absolute;
 +
background: -webkit-linear-gradient(top, #f99, #ff1a1a);
 +
background: linear-gradient(to bottom, #f99, #ff1a1a);
 +
top: -100%;
 +
left: 0;
 +
width: 100%;
 +
height: 200%;
 +
-webkit-transition: all 600ms ease;
 +
transition: all 600ms ease;
 +
}
 +
.button_main5:hover .btn-bg {
 +
top: 0;
 +
}
 +
/*************************five-end*************/
 +
 +
/************************************************************************************/
 +
/***********************************Buttons-end**************************************/
 +
/************************************************************************************/
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
/************************************************************************************/
 +
/***********************************dropdown menu************************************/
 +
/************************************************************************************/
 +
 +
.dropdown {
 +
position: fixed;
 +
top: 10px;
 +
display: inline-block;
 +
float: left;
 +
z-index: 999;
 +
}
 +
.dropdown nav {
 +
max-height: 0px;
 +
overflow: hidden;
 +
-webkit-transition: all 600ms ease;
 +
transition: all 600ms ease;
 +
}
 +
.dropdown nav a {
 +
display: block;
 +
}
 +
.dropdown:hover nav {
 +
max-height: 999px;
 +
}
 +
.dropdown a {
 +
width: 155px;
 +
display: inline-block;
 +
background: #33f;
 +
background:rgba(0,0,0,0.4);
 +
color: #fff;
 +
text-decoration: none;
 +
text-align: center;
 +
padding: 1em 2em;
 +
font-family: sans-serif;
 +
}
 +
 +
.dropdown a:hover {
 +
background: #33f;
 +
background:rgba(0,0,0,0.8);
 +
}
 +
/*************************one*****************************/
 +
.sub1 {
 +
overflow: hidden;
 +
display: -webkit-box;
 +
display: -webkit-flex;
 +
display: -ms-flexbox;
 +
display: flex;
 +
max-width: 0;
 +
max-height: 50px;
 +
-webkit-transition: all 600ms ease;
 +
transition: all 600ms ease;
 +
}
 +
.sub1 a {
 +
background: #ffb733;
 +
background:rgba(255,183,51,0.4);
 +
}
 +
.main1 {
 +
display: -webkit-box;
 +
display: -webkit-flex;
 +
display: -ms-flexbox;
 +
display: flex;
 +
}
 +
 +
.main1 a {
 +
width: 140px;
 +
}
 +
 +
.main1:hover .sub1 {
 +
max-width: 999px;
 +
max-height: 50px;
 +
}
 +
.btn-bg1 {
 +
background: #ffb733;
 +
background:rgba(255,183,51,0.4);
 +
height: 50px;
 +
width: 14px;
 +
}
 +
 +
.sub1 a:hover {
 +
background: #ffb733;
 +
background:rgba(255,183,51,0.8);
 +
}
 +
/*************************one*****************************/
 +
/*************************two*****************************/
 +
.sub2 {
 +
overflow: hidden;
 +
display: -webkit-box;
 +
display: -webkit-flex;
 +
display: -ms-flexbox;
 +
display: flex;
 +
max-width: 0;
 +
-webkit-transition: all 600ms ease;
 +
transition: all 600ms ease;
 +
}
 +
.sub2 a {
 +
background: #00a600;
 +
background:rgba(0,166,0,0.4);
 +
}
 +
.main2 {
 +
display: -webkit-box;
 +
display: -webkit-flex;
 +
display: -ms-flexbox;
 +
display: flex;
 +
}
 +
 +
.main2 a {
 +
width: 140px;
 +
}
 +
 +
.main2:hover .sub2 {
 +
max-width: 999px;
 +
max-height: 50px;
 +
}
 +
.btn-bg2 {
 +
background: #00a600;
 +
background:rgba(0,166,0,0.4);
 +
height: 50px;
 +
width: 14px;
 +
}
 +
 +
.sub2 a:hover {
 +
background: #ffb733;
 +
background:rgba(0,166,0,0.8);
 +
}
 +
/*************************two*****************************/
 +
/*************************three*****************************/
 +
.sub3 {
 +
overflow: hidden;
 +
display: -webkit-box;
 +
display: -webkit-flex;
 +
display: -ms-flexbox;
 +
display: flex;
 +
max-width: 0;
 +
-webkit-transition: all 600ms ease;
 +
transition: all 600ms ease;
 +
}
 +
.sub3 a {
 +
background: #ff1a1a;
 +
background:rgba(255,26,26,0.4);
 +
}
 +
.main3 {
 +
display: -webkit-box;
 +
display: -webkit-flex;
 +
display: -ms-flexbox;
 +
display: flex;
 +
}
 +
 +
.main3 a {
 +
width: 140px;
 +
}
 +
 +
.main3:hover .sub3 {
 +
max-width: 999px;
 +
max-height: 50px;
 +
}
 +
.btn-bg3 {
 +
background: #ff1a1a;
 +
background:rgba(255,26,26,0.4);
 +
height: 50px;
 +
width: 14px;
 +
}
 +
 +
.sub3 a:hover {
 +
background: #ffb733;
 +
background:rgba(255,26,26,0.8);
 +
}
 +
/*************************three*****************************/
 +
/*************************four*****************************/
 +
.sub4 {
 +
overflow: hidden;
 +
display: -webkit-box;
 +
display: -webkit-flex;
 +
display: -ms-flexbox;
 +
display: flex;
 +
max-width: 0;
 +
-webkit-transition: all 600ms ease;
 +
transition: all 600ms ease;
 +
}
 +
.sub4 a {
 +
background: #a600a6;
 +
background:rgba(166,0,166,0.4);
 +
}
 +
.main4 {
 +
display: -webkit-box;
 +
display: -webkit-flex;
 +
display: -ms-flexbox;
 +
display: flex;
 +
}
 +
 +
.main4 a {
 +
width: 140px;
 +
}
 +
 +
.main4:hover .sub4 {
 +
max-width: 999px;
 +
max-height: 50px;
 +
}
 +
.btn-bg4 {
 +
background: #a600a6;
 +
background:rgba(166,0,166,0.4);
 +
height: 50px;
 +
width: 14px;
 +
}
 +
 +
.sub4 a:hover {
 +
background: #ffb733;
 +
background:rgba(166,0,166,0.8);
 +
}
 +
/*************************four*****************************/
 +
/*************************five*****************************/
 +
.sub5 {
 +
overflow: hidden;
 +
display: -webkit-box;
 +
display: -webkit-flex;
 +
display: -ms-flexbox;
 +
display: flex;
 +
max-width: 0;
 +
-webkit-transition: all 600ms ease;
 +
transition: all 600ms ease;
 +
}
 +
.sub5 a {
 +
background: #0000FF;
 +
background:rgba(0,0,255,0.4);
 +
}
 +
.main5 {
 +
display: -webkit-box;
 +
display: -webkit-flex;
 +
display: -ms-flexbox;
 +
display: flex;
 +
}
 +
 +
.main5 a {
 +
width: 140px;
 +
}
 +
 +
.main5:hover .sub5 {
 +
max-width: 999px;
 +
max-height: 50px;
 +
}
 +
.btn-bg5 {
 +
background: #0000FF;
 +
background:rgba(0,0,255,0.4);
 +
height: 50px;
 +
width: 14px;
 +
}
 +
 +
.sub5 a:hover {
 +
background: #0000FF;
 +
background:rgba(0,0,255,0.8);
 +
}
 +
/*************************five*****************************/
 +
 +
/************************************************************************************/
 +
/***********************************dropdown menu-end********************************/
 +
/************************************************************************************/
 +
</style>
 +
 +
<link rel="stylesheet" href="css/style.css"/>
 +
<title>Team: Technion 2015</title>
 +
</head>
 +
<body>
 +
 
 +
<header>
 +
<!--<a href="#" ID=igem_login>Log in</a>-->
 +
 +
<img src="https://static.igem.org/mediawiki/2015/f/fc/Team_Technion_Logo.png" alt="Team Logo" id="team_logo" />
 +
 
 +
<div class="main_menu">
 +
<nav>
 +
<a href="#">
 +
<button class="button_main1">
 +
<span class="btn-text">Project</span>
 +
<span class="btn-bg"></span>
 +
</button>
 +
</a>
 +
<a href="#">
 +
<button class="button_main2">
 +
<span class="btn-text">Lab Notebook</span>
 +
<span class="btn-bg"></span>
 +
</button>
 +
</a>
 +
<a href="#">
 +
<button class="button_main3">
 +
<span class="btn-text">Judging Criteria</span>
 +
<span class="btn-bg"></span>
 +
</button>
 +
</a>
 +
<a href="#">
 +
<button class="button_main4">
 +
<span class="btn-text">Human Practices</span>
 +
<span class="btn-bg"></span>
 +
</button>
 +
</a>
 +
<a href="#">
 +
<button class="button_main5">
 +
<span class="btn-text">Team</span>
 +
<span class="btn-bg"></span>
 +
</button>
 +
</a>
 +
</nav>
 +
</div>
 +
</header>
 +
 
 +
<!---------------------------------------dropdown menu----------------------------------->
 +
<div class="dropdown">
 +
<a href="#">
 +
<img src="https://static.igem.org/mediawiki/2015/c/c1/Team_Technion_Navbar_Home.png" alt="Home" id="home_nav" />
 +
<div id="home_text">Navigate</div>
 +
</a>
 +
<nav>
 +
<div class="main1">
 +
<a href="#">Project</a>
 +
<span class="btn-bg1"></span>
 +
<div class="sub1">
 +
<a href="#">sublink1</a>
 +
<a href="#">sublink2</a>
 +
<a href="#">sublink3</a>
 +
</div>
 +
</div>
 +
<div class="main2">
 +
<a href="#">Lab Notebook</a>
 +
<span class="btn-bg2"></span>
 +
<div class="sub2">
 +
<a href="#">sublink1</a>
 +
<a href="#">sublink2</a>
 +
<a href="#">sublink3</a>
 +
</div>
 +
</div>
 +
<div class="main3">
 +
<a href="#">Judging Criteria</a>
 +
<span class="btn-bg3"></span>
 +
<div class="sub3">
 +
<a href="#">sublink1</a>
 +
<a href="#">sublink2</a>
 +
<a href="#">sublink3</a>
 +
</div>
 +
</div>
 +
<div class="main4">
 +
<a href="#">Human Practices</a>
 +
<span class="btn-bg4"></span>
 +
<div class="sub4">
 +
<a href="#">sublink1</a>
 +
<a href="#">sublink2</a>
 +
<a href="#">sublink3</a>
 +
</div>
 +
</div>
 +
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<b>Our team is developing a treatment for male pattern baldness.</b>
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Hair loss affects roughly 61 million people in the United States.  More than 95% of hair thinning in men is attributed to male pattern baldness, or androgenetic alopecia.  The hair loss product market is a multi-billion dollar market: in the United States alone, this market’s annual revenue is 7 billion dollars, with 3.5% annual growth rate from 2007 to 2012, yet a sufficient solution has yet to be made available. 
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The trigger for androgenetic alopecia is believed to be dihydrotestosterone (DHT), a derivative of testosterone.
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This summer, we are engineering Bacillus Subtilis, a model organism for a bacterium found naturally on the scalp, to secrete 3α-hydroxysteroid dehydrogenase (3-α-HSD).  This enzyme reduces DHT to a non-steroidically active compound, using NADPH and NADH as cofactors.
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Our project consists of 3 main parts: 3-α-HSD secretion by the bacterium, engineered bacterium for NADPH overproduction, and a comb as a tool to create a user-friendly experience.
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By synchronizing these parts, we hope to be able to show convincing evidence of DHT breakdown, providing a promising platform for treatment of male pattern baldness!
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Revision as of 17:42, 14 July 2015

Team: Technion 2015

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Hello! Welcome to our temporary wiki page!

Our team is developing a treatment for male pattern baldness.

Hair loss affects roughly 61 million people in the United States. More than 95% of hair thinning in men is attributed to male pattern baldness, or androgenetic alopecia. The hair loss product market is a multi-billion dollar market: in the United States alone, this market’s annual revenue is 7 billion dollars, with 3.5% annual growth rate from 2007 to 2012, yet a sufficient solution has yet to be made available. The trigger for androgenetic alopecia is believed to be dihydrotestosterone (DHT), a derivative of testosterone. This summer, we are engineering Bacillus Subtilis, a model organism for a bacterium found naturally on the scalp, to secrete 3α-hydroxysteroid dehydrogenase (3-α-HSD). This enzyme reduces DHT to a non-steroidically active compound, using NADPH and NADH as cofactors.

Our project consists of 3 main parts: 3-α-HSD secretion by the bacterium, engineered bacterium for NADPH overproduction, and a comb as a tool to create a user-friendly experience. By synchronizing these parts, we hope to be able to show convincing evidence of DHT breakdown, providing a promising platform for treatment of male pattern baldness!

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