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| − | <h2> Welcome to iGEM 2015! </h2> | + | <center> |
| − | <p>Your team has been approved and you are ready to start the iGEM season! </p> | + | <h1> Opium War </h1> |
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| | + | Project Description |
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| − | <h4>Before you start: </h4>
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| − | <p> Please read the following pages:</p>
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| − | <ul>
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| − | <li> <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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| − | <li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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| − | </ul>
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| − | <div class="highlightBox"> | + | <p> As is known to all, drugs are extremely harmful both to our mental and physical health. Among different kinds of drugs, opioid drug is one of the most widely used and hazardous drugs, which includes heroin and morphine. There are several withdraw methods being applied currently, such as natural withdraw, non-drug withdraw and drug withdraw method, each of which has its side effects. Our project is exploring a brand new way to help morphine addicts rehabilitate and prevent relapse. </p> |
| − | <h4> Styling your wiki </h4>
| + | <p>Cell-derived exosomes have been demonstrated to be efficient carriers of small RNAs to neighbouring or distant cells. Hopefully, we intend to employ modified exosomes expressing the neuron-specific rabies viral glycoprotein (RVG) peptide on the membrane surface to deliver opioid receptor mu (MOR) siRNA into the brain to treat morphine addiction.</p> |
| − | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p> | + | <p>Through targeting experiment, we have demonstrated that siRNA can be efficiently packaged into exosomes and specifically delivered to N2a neurons(in vitro) or mice brain tissues(in vivo). Functionally, siRNA-loaded RVG exosomes significantly reduced MOR mRNA and protein levels in mice. What’s more, we are thrilled to find out that treatment of MOR siRNA delivered by RVG exosomes strongly inhibited morphine relapse by down-regulating the expression of MOR receptor. These experiments demonstrate that siRNA RVG exosomes can specifically target to brain tissue and efficiently prevent morphine relapse. </p> |
| − | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p> | + | |
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| − | <h4> Editing your wiki </h4>
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| − | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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| − | <p> <a href="https://2015.igem.org/wiki/index.php?title=Team:NJU-China&action=edit"> Click here to edit this page! </a></p>
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| − | <p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>
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| − | <h4>Templates </h4>
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| − | <p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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| − | <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>
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| − | <h4>Tips</h4>
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| − | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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| − | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
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| − | <li>Be clear about what you are doing and how you plan to do this.</li>
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| − | <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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| − | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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| − | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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| − | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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| − | <li>Have lots of fun! </li>
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| − | </ul>
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| − | <h4>Inspiration</h4>
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| − | <p> You can also view other team wikis for inspiration! Here are some examples:</p>
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| − | <ul>
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| − | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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| − | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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| − | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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| − | </ul>
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| − | <h4> Uploading pictures and files </h4>
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| − | <p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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| − | When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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| − | <a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
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| − | </div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
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Add a banner to your wiki!
You can make the image 980px by 200px
Remember to call the file: "Team_NJU-China_banner.jpg"
Opium War
Project Description
As is known to all, drugs are extremely harmful both to our mental and physical health. Among different kinds of drugs, opioid drug is one of the most widely used and hazardous drugs, which includes heroin and morphine. There are several withdraw methods being applied currently, such as natural withdraw, non-drug withdraw and drug withdraw method, each of which has its side effects. Our project is exploring a brand new way to help morphine addicts rehabilitate and prevent relapse.
Cell-derived exosomes have been demonstrated to be efficient carriers of small RNAs to neighbouring or distant cells. Hopefully, we intend to employ modified exosomes expressing the neuron-specific rabies viral glycoprotein (RVG) peptide on the membrane surface to deliver opioid receptor mu (MOR) siRNA into the brain to treat morphine addiction.
Through targeting experiment, we have demonstrated that siRNA can be efficiently packaged into exosomes and specifically delivered to N2a neurons(in vitro) or mice brain tissues(in vivo). Functionally, siRNA-loaded RVG exosomes significantly reduced MOR mRNA and protein levels in mice. What’s more, we are thrilled to find out that treatment of MOR siRNA delivered by RVG exosomes strongly inhibited morphine relapse by down-regulating the expression of MOR receptor. These experiments demonstrate that siRNA RVG exosomes can specifically target to brain tissue and efficiently prevent morphine relapse.