Difference between revisions of "Team:Paris Saclay/Notebook/July/8"

(Created page with "=Wednesday 8th July= ==Lab Work== ====Transformation==== ''by Audrey'' R0051 On LB + Ampicillin 100ug/mL ====Digestion==== ''by Pauline, Coralie'' 10uL of our plasmid with p...")
 
(→‎Wednesday 8th July)
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R0051  
 
R0051  
 
On LB + Ampicillin 100ug/mL  
 
On LB + Ampicillin 100ug/mL  
 +
 +
====Rehydratation====
 +
''by Seong Koo, Johan''
 +
 +
R0051 from different years
  
 
====Digestion====
 
====Digestion====
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1uL PstI
 
1uL PstI
 
2uL buffer 10x FD
 
2uL buffer 10x FD
 +
 +
*J23101
 +
*J23106
 +
*J23117
  
 
10uL of our plasmid with gene
 
10uL of our plasmid with gene
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2uL buffer 10x FD
 
2uL buffer 10x FD
 
After this step, we separate the sequence we need from the sequence we doesn't with electrophoresis
 
After this step, we separate the sequence we need from the sequence we doesn't with electrophoresis
 +
 +
*K115017
 +
*I13504
  
 
====Electrophoresis====
 
====Electrophoresis====
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Migration 0,06A 80V  
 
Migration 0,06A 80V  
  
 +
*K115017
 +
*I13504
 +
 +
====Ligation====
 +
''by Coralie, Audrey''
 +
 +
2,5uL J23117
 +
15uL I13504
 +
1uL Ligase
 +
2uL buffer 10x
 +
 +
1,5uL J23106
 +
15,5uL I13504
 +
1uL Ligase
 +
2uL buffer 10x
 +
 +
2uL J23101
 +
14,5uL I13504
 +
1uL Ligase
 +
2uL buffer 10x
 +
 +
Waiting all the night under 16°C
  
 
'''Members present:'''
 
'''Members present:'''
 
* Instructors and advisors: Alice.
 
* Instructors and advisors: Alice.
 
* Students: Johan, Seong Koo, Audrey, Coralie, Pauline
 
* Students: Johan, Seong Koo, Audrey, Coralie, Pauline

Revision as of 10:44, 15 July 2015

Wednesday 8th July

Lab Work

Transformation

by Audrey

R0051 On LB + Ampicillin 100ug/mL

Rehydratation

by Seong Koo, Johan

R0051 from different years

Digestion

by Pauline, Coralie

10uL of our plasmid with promotor 1uL SpeI 1uL PstI 2uL buffer 10x FD

  • J23101
  • J23106
  • J23117

10uL of our plasmid with gene 1uL XbaI 1uL PstI 2uL buffer 10x FD After this step, we separate the sequence we need from the sequence we doesn't with electrophoresis

  • K115017
  • I13504

Electrophoresis

by Johan, Coralie, Seong Koo, Pauline

Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET Migration 0,06A 80V

  • K115017
  • I13504

Ligation

by Coralie, Audrey

2,5uL J23117 15uL I13504 1uL Ligase 2uL buffer 10x

1,5uL J23106 15,5uL I13504 1uL Ligase 2uL buffer 10x

2uL J23101 14,5uL I13504 1uL Ligase 2uL buffer 10x

Waiting all the night under 16°C

Members present:

  • Instructors and advisors: Alice.
  • Students: Johan, Seong Koo, Audrey, Coralie, Pauline