Revision as of 14:24, 15 July 2015

Tuesday 7th July

Back in Lab

Verification of our plasmids

by Coralie, Johan, Seong Koo, Audrey, Pauline

Biobricks verification:

• K115017
• C0040
• I13602
• J23101
• J23117
• J23106
• I13504

Extraction

With the Nucleospin Kit from Macherey Nagel

Digestion by NotI:

Stock solution preparation :

• 124µL H2O
• 16µL buffer FastDigest 10x
• 4µL NotI

We use 18µL from this solution with 2µL plasmid solution

Incubation 1h30 at 37°C

Electrophoresis

by Johan, Audrey, Pauline

Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET Migration 0,06A 80V

New culture

by Pauline, Audrey

New liquid culture of:

• K1493504

2x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony. We incubate cultures at 37°C, ON.

Members present:

• Instructors and advisors: Alice.
• Students: Johan, Seong Koo, Audrey, Coralie, Pauline