Difference between revisions of "Team:Paris Saclay/Notebook/July/15"
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Incubation 3h at 4°C | Incubation 3h at 4°C | ||
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''by Seong Koo'' | ''by Seong Koo'' | ||
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Biobrick: R0051 | Biobrick: R0051 | ||
Revision as of 09:32, 17 July 2015
Contents
Wednesday 15th July
Back in Lab
Observation of our plates
by Coralie and Johan
- Transformations from the 07/10/2015
We can observe that S03518, B0030 and B0015 are Ok. But K1399005 didn't grow. We think that we make a mistake when we rehydrated the plasmid.
- Transformations from the 07/09/2015
We can observe that J23101 + I13504 and J23106 + I13504 are yellow when we expose them to the white light. But J23117 + I13504 don't seem to be yellow on the white light.
New culture
by Coralie, Johan and Seong Koo
New liquid culture of:
- Transformations from the 07/09/2015:
- J23101 + I13504
- J23106 + I13504
- J23117 + I13504
- B0015
- B0030
- S03518
2x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony.
We incubate cultures at 37°C, ON.
Ligation:
by Pauline
Reaction mix:
- J23101: 1µL
- K115017: 10µL
- Buffer (10x): 2µL
- Ligase: 1µL
- H2O: 6µL
Incubation 3h at 4°C
Transformation
by Seong Koo
Biobricks:
- K139905
- K1399019
- K1399023
Ligation product:
- J23101 + K115017
Plasmid extraction
Biobrick: R0051 Culture form the 07/10/2015 With the Nucleospin Kit from Macherey Nagel
Members present:
- Instructors and advisors: Alice.
- Students: Johan, Seong Koo, Coralie, Pauline