Difference between revisions of "Team:Reading/Notebook"

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<p> <b> 07/07/15 </b> </p>
 
<p> <b> 07/07/15 </b> </p>
 
<li>Flask 5 cultured containing 10ul .6803 WT in 100ml BG-11. This flask will be used for preliminary growth curve measurements. The flask was placed on the shaker with flasks 2,3 and 4. </li>
 
<li>Flask 5 cultured containing 10ul .6803 WT in 100ml BG-11. This flask will be used for preliminary growth curve measurements. The flask was placed on the shaker with flasks 2,3 and 4. </li>
−
<!-- Table of growth curve measurements -->
 
 
<table style="width:50%">
 
<table style="width:50%">
 
<tr> <th> Date </th> <th> OD<sub>750</sub> </th> <th> Estimated Cells per ml </th> </tr>
 
<tr> <th> Date </th> <th> OD<sub>750</sub> </th> <th> Estimated Cells per ml </th> </tr>
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<p> <b> 13/07/15 </b> </p>  
 
<p> <b> 13/07/15 </b> </p>  
 
<p> Estimating the Cells per ml for the flask from 29/06 1a.u at 750nm = 1.6x10<sup>8</sup> <li> OD<sub>750</sub> = 0.028 </li> So <li> 0.028x1.6x10<sup>8</sup> = 4.48x10<sup>6</sup> cells per ml </li> </p>  
 
<p> Estimating the Cells per ml for the flask from 29/06 1a.u at 750nm = 1.6x10<sup>8</sup> <li> OD<sub>750</sub> = 0.028 </li> So <li> 0.028x1.6x10<sup>8</sup> = 4.48x10<sup>6</sup> cells per ml </li> </p>  
 +
<p> OD<sub>750</sub> measurement and cells per ml estimated for flasks cultured on 02/07 </p>
 
<table style="width:50%">
 
<table style="width:50%">
 
<tr> <th> Date </th> <th> Flask No. </th> <th> OD<sub>750</sub> </th> <th> Estimated Cells per ml </th> </tr>
 
<tr> <th> Date </th> <th> Flask No. </th> <th> OD<sub>750</sub> </th> <th> Estimated Cells per ml </th> </tr>
 
<tr> <td rowspan="3"> 13/07 </td> <td> 2 </td> <td> 0.006 </td> <td> 9.6x10<sup>5</sup> </td> </tr>  
 
<tr> <td rowspan="3"> 13/07 </td> <td> 2 </td> <td> 0.006 </td> <td> 9.6x10<sup>5</sup> </td> </tr>  
 
<tr> <td> 3 </td> <td> 0.012 </td> <td> 1.92x10<sup>6</sup> </td> </tr>
 
<tr> <td> 3 </td> <td> 0.012 </td> <td> 1.92x10<sup>6</sup> </td> </tr>
−
<tr> <td> 4 </td> <td> 0.015 </td> <td> 2.4x10<sup>6</sup> </td> </tr> </table>
+
<tr> <td> 4 </td> <td> 0.015 </td> <td> 2.4x10<sup>6</sup> </td> </tr>  
 +
<tr> <td rowspan="3"> 14/07 </td> <td> 2 </td> <td> 0.006 </td> <td> 9.6x10<sup>5</sup> </td> </tr>
 +
<tr> <td> 3 </td> <td> 0.018 </td> <td> 2.88x10<sup>6</sup> </td> </tr>
 +
<tr> <td> 4 </td> <td> 0.017 </td> <td> 2.72x10<sup>6</sup> </td> </tr>
 +
<tr> <td rowspan="3"> 15/07 </td> <td> 2 </td> <td> 0.006 </td> <td> 9.6x10<sup>5</sup> </td> </tr>
 +
<tr> <td> 3 </td> <td> 0.021 </td> <td> 3.36x10<sup>6</sup> </td> </tr>
 +
<tr> <td> 4 </td> <td> 0.018 </td> <td> 2.88x10<sup>6</sup> </td> </tr>
 +
<tr> <td rowspan="3"> 16/07 </td> <td> 2 </td> <td> 0.001 </td> <td> 1.6x10<sup>5</sup> </td> </tr>
 +
<tr> <td> 3 </td> <td> 0.020 </td> <td> 3.2x10<sup>6</sup> </td> </tr>
 +
<tr> <td> 4 </td> <td> 0.019 </td> <td> 3.04x10<sup>6</sup> </td> </tr>
 +
<tr> <td rowspan="3"> 17/07 </td> <td> 2 </td> <td> 0.001 </td> <td> 1.6x10<sup>5</sup> </td> </tr>
 +
<tr> <td> 3 </td> <td> 0.040 </td> <td> 6.4x10<sup>6</sup> </td> </tr>
 +
<tr> <td> 4 </td> <td> 0.027 </td> <td> 4.32x10<sup>6</sup> </td> </tr>
 +
</table>
 
<p> <b> 14/07/15 </b> </p>
 
<p> <b> 14/07/15 </b> </p>
 
<p> The lamp safety testing was completed so our flasks were placed under the white light bulb </p>
 
<p> The lamp safety testing was completed so our flasks were placed under the white light bulb </p>

Revision as of 19:07, 17 July 2015



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Lab Book

Dates are in the UK format DD/MM/YY

29/06/15

  • Cultured a 250ml conical flask using 100 ml BG-11 and 5 loopfuls of .6803 WT.
  • Cultured 5 streak plates of .6803 on solid BG-11

    • All were placed on the windowsill in our lab in natural light

    02/07/15

  • Three new 250ml conical flasks were set up on the shaker (42rpm). Each contained 5 loopfuls of .6803 WT and 100ml of BG-11. They were exposed to 24hr light by the overhead lights in the lab.

    • 07/07/15

  • Flask 5 cultured containing 10ul .6803 WT in 100ml BG-11. This flask will be used for preliminary growth curve measurements. The flask was placed on the shaker with flasks 2,3 and 4.
    • Date OD750 Estimated Cells per ml
    07/07 0.000 0
    08/07 0.003 4.8x105
    09/07 0.005 8x105
    10/07 0.005 8x105
    13/07 0.005 8x105
    14/07 0.112 1.792x107
    15/07 0.008 1.28x106
    16/07 0.009 1.44x106
    17/07 0.03 4.8x106

    10/07/15

    2 Litres of water collected from Whiteknights Lake on campus for SODIS, method outlines in protocols.

    13/07/15

    Estimating the Cells per ml for the flask from 29/06 1a.u at 750nm = 1.6x108

  • OD750 = 0.028
    • So
  • 0.028x1.6x108 = 4.48x106 cells per ml

    • OD750 measurement and cells per ml estimated for flasks cultured on 02/07

    Date Flask No. OD750 Estimated Cells per ml
    13/07 2 0.006 9.6x105
    3 0.012 1.92x106
    4 0.015 2.4x106
    14/07 2 0.006 9.6x105
    3 0.018 2.88x106
    4 0.017 2.72x106
    15/07 2 0.006 9.6x105
    3 0.021 3.36x106
    4 0.018 2.88x106
    16/07 2 0.001 1.6x105
    3 0.020 3.2x106
    4 0.019 3.04x106
    17/07 2 0.001 1.6x105
    3 0.040 6.4x106
    4 0.027 4.32x106

    14/07/15

    The lamp safety testing was completed so our flasks were placed under the white light bulb

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