Difference between revisions of "Team:Reading/Notebook"

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<h2> Lab Book</h2>
 
<h2> Lab Book</h2>
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<p> Dates are in the UK format DD/MM/YY </p>
+
<p> Dates are in the UK format DD/MM/YY. 6803 refers to the cyanobacterium used - Synechocystis sp. PCC 6803 </p>
 
<p> <b> 29/06/15 </b> </p>
 
<p> <b> 29/06/15 </b> </p>
 
<li> Cultured a 250ml conical flask using 100 ml BG-11 and 5 loopfuls of 6803 Wild Type (WT). </li>
 
<li> Cultured a 250ml conical flask using 100 ml BG-11 and 5 loopfuls of 6803 Wild Type (WT). </li>
 
<li> Cultured 5 streak plates of 6803 WT on BG-11 Agar </li>
 
<li> Cultured 5 streak plates of 6803 WT on BG-11 Agar </li>
−
<p> All were placed on the windowsill in our lab in natural light </p>
+
<p> All were placed on the windowsill in our lab under natural light </p>
 
<!-- Insert image of flask and plates on windowsill -->
 
<!-- Insert image of flask and plates on windowsill -->
 
<p> <b> 02/07/15 </b> </p>
 
<p> <b> 02/07/15 </b> </p>
−
<li> Three new 250ml conical flasks were set up on the shaker (42rpm). Each contained 5 loopfuls of .6803 WT and 100ml of BG-11. They were exposed to 24hr light by the overhead lights in the lab. </li>   
+
<li> Three new 250ml conical flasks were set up on the shaker (42rpm). Each contained 5 loopfuls of 6803 WT and 100ml of BG-11. They were exposed to the overhead lights in the lab 24/7. </li>   
 
<p> <b> 07/07/15 </b> </p>
 
<p> <b> 07/07/15 </b> </p>
−
<li>Flask 5 cultured containing 10ul .6803 WT in 100ml BG-11. This flask will be used for preliminary growth curve measurements. The flask was placed on the shaker with flasks 2,3 and 4. </li>
+
<li>Flask 5 cultured containing 10ul 6803 WT in 100ml BG-11. This flask will be used for preliminary growth curve measurements. The flask was placed on the shaker with flasks 2,3 and 4. </li>
 
<table style="width:50%">
 
<table style="width:50%">
 
<tr> <th> Date </th> <th> OD<sub>750</sub> </th> <th> Estimated Cells per ml </th> </tr>
 
<tr> <th> Date </th> <th> OD<sub>750</sub> </th> <th> Estimated Cells per ml </th> </tr>
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<tr> <td> 15/07 </td> <td> 0.008 </td> <td> 1.28x10<sup>6</sup> </td> </tr>
 
<tr> <td> 15/07 </td> <td> 0.008 </td> <td> 1.28x10<sup>6</sup> </td> </tr>
 
<tr> <td> 16/07 </td> <td> 0.009 </td> <td> 1.44x10<sup>6</sup> </td> </tr>
 
<tr> <td> 16/07 </td> <td> 0.009 </td> <td> 1.44x10<sup>6</sup> </td> </tr>
−
<tr> <td> 17/07 </td> <td> 0.03 </td> <td> 4.8x10<sup>6</sup> </td> </tr>
+
<tr> <td> 17/07 </td> <td> 0.030 </td> <td> 4.8x10<sup>6</sup> </td> </tr>
 
</table>
 
</table>
 
<p> <b> 10/07/15 </b> </p>  
 
<p> <b> 10/07/15 </b> </p>  
 
<p> 2 Litres of water collected from Whiteknights Lake on campus for SODIS, method outlines in protocols. </p>
 
<p> 2 Litres of water collected from Whiteknights Lake on campus for SODIS, method outlines in protocols. </p>
 
<p> <b> 13/07/15 </b> </p>  
 
<p> <b> 13/07/15 </b> </p>  
−
<p> Estimating the Cells per ml for the flask from 29/06 1a.u at 750nm = 1.6x10<sup>8</sup> <li> OD<sub>750</sub> = 0.028 </li> Therefore <li> 0.028x1.6x10<sup>8</sup> = 4.48x10<sup>6</sup> cells per ml </li> </p>  
+
<p> Estimating the Cells per ml for the flask from 29/06 1 a.u at 750nm = 1.6x10<sup>8</sup> <li> OD<sub>750</sub> = 0.028 </li> Therefore <li> 0.028x1.6x10<sup>8</sup> = 4.48x10<sup>6</sup> cells per ml </li> </p>  
 
<p> OD<sub>750</sub> measurement and cells per ml estimated for flasks cultured on 02/07 </p>
 
<p> OD<sub>750</sub> measurement and cells per ml estimated for flasks cultured on 02/07 </p>
 
<table style="width:50%">
 
<table style="width:50%">

Revision as of 19:41, 17 July 2015



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Lab Book

Dates are in the UK format DD/MM/YY. 6803 refers to the cyanobacterium used - Synechocystis sp. PCC 6803

29/06/15

  • Cultured a 250ml conical flask using 100 ml BG-11 and 5 loopfuls of 6803 Wild Type (WT).
  • Cultured 5 streak plates of 6803 WT on BG-11 Agar

    • All were placed on the windowsill in our lab under natural light

    02/07/15

  • Three new 250ml conical flasks were set up on the shaker (42rpm). Each contained 5 loopfuls of 6803 WT and 100ml of BG-11. They were exposed to the overhead lights in the lab 24/7.

    • 07/07/15

  • Flask 5 cultured containing 10ul 6803 WT in 100ml BG-11. This flask will be used for preliminary growth curve measurements. The flask was placed on the shaker with flasks 2,3 and 4.
    • Date OD750 Estimated Cells per ml
    07/07 0.000 0
    08/07 0.003 4.8x105
    09/07 0.005 8x105
    10/07 0.005 8x105
    13/07 0.005 8x105
    14/07 0.112 1.792x107
    15/07 0.008 1.28x106
    16/07 0.009 1.44x106
    17/07 0.030 4.8x106

    10/07/15

    2 Litres of water collected from Whiteknights Lake on campus for SODIS, method outlines in protocols.

    13/07/15

    Estimating the Cells per ml for the flask from 29/06 1 a.u at 750nm = 1.6x108

  • OD750 = 0.028
    • Therefore
  • 0.028x1.6x108 = 4.48x106 cells per ml

    • OD750 measurement and cells per ml estimated for flasks cultured on 02/07

    Date Flask No. OD750 Estimated Cells per ml
    13/07 2 0.006 9.6x105
    3 0.012 1.92x106
    4 0.015 2.4x106
    14/07 2 0.006 9.6x105
    3 0.018 2.88x106
    4 0.017 2.72x106
    15/07 2 0.006 9.6x105
    3 0.021 3.36x106
    4 0.018 2.88x106
    16/07 2 0.001 1.6x105
    3 0.020 3.2x106
    4 0.019 3.04x106
    17/07 2 0.001 1.6x105
    3 0.040 6.4x106
    4 0.027 4.32x106

    14/07/15

    The lamp safety testing was completed so our flasks were placed under the white light bulb

    What should this page have?
    • Chronological notes of what your team is doing.
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    • Mention who participated in what task.