Difference between revisions of "Team:Paris Saclay/Notebook/July/17"
(Created page with "=Thursday 16th July= ====Ligation ==== ''by Pauline'' In each tube: * 3 µL Buffer DNA pol T4 * 1µL DNA pol T4 * 4µL H2O Biobricks: * J23101 (10µL) + S03518 (12µL) * S035...") |
(→Thursday 16th July) |
||
| Line 1: | Line 1: | ||
| − | = | + | =Friday 17th July= |
Revision as of 12:25, 20 July 2015
Contents
Friday 17th July
Ligation
by Pauline
In each tube:
- 3 µL Buffer DNA pol T4
- 1µL DNA pol T4
- 4µL H2O
Biobricks:
- J23101 (10µL) + S03518 (12µL)
- S03518 (12µL) + B0015 (10µL)
- B0015 (10µL) + I13602 (12µL)
Incubation 4h, 4°C
New culture
by Coralie
- 1696: Bad::Tet
- 1320:ClpP::Cm
- 1693:z1:Spect
On LB plates, incubation ON, 37°C
Transformation
by Johan
Transformation with ligation product:
- J23101 + S03518 = K1707001
- S03518 + B0015 = K1707003
- B0015 + I13602 = K1707002
New culture on plates
by Pauline
- J23101 + I13504
- J23106 + I13504
- J23117 + I13504
- B0030
- R0051
Plasmid extraction
by Pauline and Seong-Koo
Biobricks:
- K1707000
- K1399019
- K1399005
- K1399023
Cultures from the 16/15/2015 With the Nucleospin Kit from Macherey Nagel
Members present:
- Instructors and advisors: Alice.
- Students: Johan, Seong Koo, Coralie, Pauline