Difference between revisions of "Team:UCLA/Notebook/Protein Cages/21 July 2015"
| Line 3: | Line 3: | ||
Nithin's Notes | Nithin's Notes | ||
| − | PCR to add the iGEM suffix to our PcQuad was done. We used igem prefix 1 and igem suffix 2 to run the reaction. The template DNA was obtained through the dna extraction that was done yesterday. This product was diluted to give us a 20ul tube of 1ng/ul. After running the gel, we werent able to see the band that we wanted. But, we did see the same nonspecific band that had haunted us earlier shining ever so brightly. | + | PCR to add the iGEM suffix to our PcQuad was done. We used igem prefix 1 and igem suffix 2 to run the reaction. The template DNA was obtained through the dna extraction that was done yesterday. This product was diluted to give us a 20ul tube of 1ng/ul. I loaded 500 ng of template for a 50 ul reacton. After running the gel, we werent able to see the band that we wanted. But, we did see the same nonspecific band that had haunted us earlier shining ever so brightly. |
I did change the annealing time from 25 seconds to 30 seconds for this reaction. | I did change the annealing time from 25 seconds to 30 seconds for this reaction. | ||
Revision as of 00:59, 22 July 2015
Nithin's Notes
PCR to add the iGEM suffix to our PcQuad was done. We used igem prefix 1 and igem suffix 2 to run the reaction. The template DNA was obtained through the dna extraction that was done yesterday. This product was diluted to give us a 20ul tube of 1ng/ul. I loaded 500 ng of template for a 50 ul reacton. After running the gel, we werent able to see the band that we wanted. But, we did see the same nonspecific band that had haunted us earlier shining ever so brightly.
I did change the annealing time from 25 seconds to 30 seconds for this reaction.
Lane 1: Ladder
Lane 2-4: 25ul of the same reaction with same conditions.