Revision as of 08:56, 22 July 2015

Monday 20th July

Lab Work

New Culture

by Audrey and Coralie

BBa_K1707001
BBa_K1707002
BBa_K1707003
BBa_R0051
BBa_K098997

1696-Bad::Tetracyclin
1320-ClpP::Chloramphenicol
1693-MG1655Z1: SpectinomycinR

Re-hydration and transformation

by Pauline

BBa_C0051
BBa_E0240
BBa_J06702

Soil contamination experiment

by Johan and Coralie We use a soil extract which was prepared by suspending 1g of soil in 5mL of steril water. We made 5 dilutions of the supernatant: 10^-2, 10^-3, 10^-4, 10^-5 and 10^-6. We streaked 100µL of each dilution in different plates:

LB medium:
- Without any antibiotic
- 100ng/µL Spectinomycin
- 20 ng/µL Chloramphenicol
- 10ng/µL Tetracycline
Mac Conkey medium:
- Without any antibiotic
- 100ng/µL Spectinomycin
- 20 ng/µL Chloramphenicol
- 10ng/µL Tetracycline

Incubation ON, 37°C

Member present:

Instructors: Alice
Students: Coralie, Pauline, Audrey, Seong-Koo and Johan

Back to the calendar

@@ Line 23: / Line 23: @@
 ===Soil contamination experiment===
+'' by Johan and Coralie''
+We use a soil extract which was prepared by suspending 1g of soil in 5mL of steril water. We made 5 dilutions of the supernatant: 10^-2, 10^-3, 10^-4, 10^-5 and 10^-6.
+We streaked 100µL of each dilution in different plates:
+* LB medium:
+** Without any antibiotic
+** 100ng/µL Spectinomycin
+** 20 ng/µL Chloramphenicol
+** 10ng/µL Tetracycline
+* Mac Conkey medium:
+** Without any antibiotic
+** 100ng/µL Spectinomycin
+** 20 ng/µL Chloramphenicol
+** 10ng/µL Tetracycline
+Incubation ON, 37°C

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