Revision as of 09:06, 22 July 2015

Tuesday 21st July

Pauline PCR Mix:

2µL of DNA in each tube:

18µL from the mix in each tube

Cycle:

by Pauline

by Coralie and Audrey

Member present:

@@ Line 2: / Line 2: @@
 =Tuesday 21st July=
 ==Lab Work==
-===Titre de l'Experience===
+===PCR===
-''by coffee boy''
+''Pauline''
+PCR Mix:
+* Buffer (5x): 30µL
+* Forward Primer: 7,5 µL
+* Reverse Primer: 7,5µL
+* dNTP: 3µL
+* DNApol GoTaq: 0,75µL
+* MgCl2: 15µL
+* H2O: 63,75µL
+µL of DNA in each tube:
+* BBa_J23101
+* BBa_K1707000
+µL from the mix in each tube
+Cycle:
+* Initiation: 95°C, 2min
+* Cycle: 95°C, 1min - 61°C, 1min - 72°C, 20sec
+* Termination: 72°C, 5min
+===New culture===
+''by Pauline''
+* BBa_C0051
+* BBa_E0240
+* BBa_J06702
+===Plasmid extraction===
+'' by Coralie and Audrey''
+* BBa_K1707001 #1 and #2
+* BBa_K1707002 #1 and #2
+* BBa_K1707003 #1 and #2
+* BBa_R0051
+* BBa_K098997
-Voilà, ça c'est le standard pour le notebook. Pour modifier cette page, allez au ménou '''wiki tools''' en haut à gauche, puis sur '''Edit this page'''. Si vous avez des questions, n'hésitez pas à me contacter. ''Bien évidemment ce text doit être supprimer une fois le notebook rempli :) Bonnes manips!
 '''Member present:'''
-* Instructors:
+* Instructors: Alice
-* Students:
+* Students: Pauline, Coralie, Audrey, Johan, Seong-Koo
 {{Team:Paris_Saclay/notebook_footer}}