Difference between revisions of "Team:Reading/Notebook"

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<p> Flask 2 was discarded as it was not showing growth. We held a full team meeting with and itinerary and everything. We also received most of the lab equipment we had ordered. Should be able to start doing interesting things now. </p>   
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<p> Flask 2 was discarded as it was not showing growth. We held a full team meeting with an itinerary and everything. We also received most of the lab equipment we had ordered. Should be able to start doing interesting things now. </p>   
  
 
<h5>What should this page have?</h5>
 
<h5>What should this page have?</h5>

Revision as of 14:09, 23 July 2015



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Lab Book

Dates are in the UK format DD/MM/YY. 6803 refers to the cyanobacterium used - Synechocystis sp. PCC 6803

29/06/15

  • Cultured a 250ml conical flask using 100 ml BG-11 and 5 loopfuls of 6803 Wild Type (WT).
  • Cultured 5 streak plates of 6803 WT on BG-11 Agar

    • All were placed on the windowsill in our lab under natural light

    02/07/15

  • Three new 250ml conical flasks were set up on the shaker (42rpm). Each contained 5 loopfuls of 6803 WT and 100ml of BG-11. They were exposed to the overhead lights in the lab 24/7.

    • 07/07/15

  • Flask 5 cultured containing 10ul 6803 WT in 100ml BG-11. This flask will be used for preliminary growth curve measurements. The flask was placed on the shaker with flasks 2,3 and 4.
    • Date OD750 Estimated Cells per ml
    07/07 0.000 0
    08/07 0.003 4.8x105
    09/07 0.005 8x105
    10/07 0.005 8x105
    13/07 0.005 8x105
    14/07 0.112 1.792x107
    15/07 0.008 1.28x106
    16/07 0.009 1.44x106
    17/07 0.030 4.8x106
    20/07 0.364 5.824x107
    21/07 0.510 8.160x107

    10/07/15

    2 Litres of water collected from Whiteknights Lake on campus for SODIS, method outlines in protocols.

    13/07/15

    Estimating the Cells per ml for the flask from 29/06 1 a.u at 750nm = 1.6x108

  • OD750 = 0.028
    • Therefore
  • 0.028x1.6x108 = 4.48x106 cells per ml

    • OD750 measurement and cells per ml estimated for flasks cultured on 02/07

    Date Flask No. OD750 Estimated Cells per ml
    13/07 2 0.006 9.6x105
    3 0.012 1.92x106
    4 0.015 2.4x106
    14/07 2 0.006 9.6x105
    3 0.018 2.88x106
    4 0.017 2.72x106
    15/07 2 0.006 9.6x105
    3 0.021 3.36x106
    4 0.018 2.88x106
    16/07 2 0.001 1.6x105
    3 0.020 3.2x106
    4 0.019 3.04x106
    17/07 2 0.001 1.6x105
    3 0.040 6.4x106
    4 0.027 4.32x106
    20/07 2 0.005 8x105
    3 0.577 9.232x107
    4 0.486 7.776x107
    21/07 2 0.001 1.6x105
    3 0.757 1.211x108
    4 0.653 1.045x108
    22/07 2 0.001 1.6x105
    3 1.049 1.678x108
    4 0.916 1.466x108
    23/07 3 1.302 2.083x108
    4 1.203 1.925x108

    14/07/15

    The lamp safety testing was completed so our flasks were placed under the white light bulb

    21/07/15

    Flasks are seriously green!

    23/07/15

    Flask 2 was discarded as it was not showing growth. We held a full team meeting with an itinerary and everything. We also received most of the lab equipment we had ordered. Should be able to start doing interesting things now.

    What should this page have?
    • Chronological notes of what your team is doing.
    • Brief descriptions of daily important events.
    • Pictures of your progress.
    • Mention who participated in what task.