Difference between revisions of "Team:Paris Saclay/Notebook/July/1"

m (1 July)
(Preparation LB Plate)
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===Preparation LB Plate===
 
===Preparation LB Plate===
''by SeongKoo and Pauline''
+
''by Seong Koo and Pauline''
  
 
22 Plates LB + Cm (20uL/mL)
 
22 Plates LB + Cm (20uL/mL)

Revision as of 09:49, 27 July 2015


July 1

Lab Work

Preparation

by Johan

Plasmid Rehydratation :

by Johan Measure fluorescence : I13504 J23117 J23106 J23101

For our project : R0051 J13602 J13522 C0040

Preparation of liquid culture of DH5a with K115017

K115017 5mL LB + 5uL Cm (20mg/mL)

Competente cells preparation

by Seong Koo, Pauline, Johan

Preparation competente cells DH5a with CaCl2 cf:protocol

15mL competente cells Aliquot the solution in chilled 1.5mL microcentrifuge tubes (500uL/tube) and frozen and store at -70°C

Preparation LB Plate

by Seong Koo and Pauline

22 Plates LB + Cm (20uL/mL) 12 Plates LB + Amp (100uL/mL)


Members present:

  • Instructors : Alice.
  • Students : Johan, Seong Koo, Pauline.

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