Difference between revisions of "Team:KU Leuven/Wetlab"

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<h2>Wet Lab</h2>
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<h2>Research</h2>
 
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Patterns are fascinating, from the veins of a leaf to the spots on a zebra, from a single cell to a whole organism. Patterns are found everywhere in nature, but how these are formed is not entirely clear. We, the KU Leuven 2015 iGEM team, decided to work on the fundamental mechanisms behind pattern formation. The way cells interact to generate a specific pattern has triggered our curiosity and added a new dimension to the way the patterns are looked upon. Our mission is to create different and astonishing biological patterns with engineered bacteria for a better understanding of nature with the prospect of applying the knowledge in industry.
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We designed a circuit capable of producing patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger this structure formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns, providing advancements in a variety of industrial processes like for example the creation of novel bio-materials. On the other hand, this fundamental project could also speed up medical research projects on among others tumor formation and tissue regeneration.
 
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<a href="https://2015.igem.org/Team:KU_Leuven/Project/About">Read more</a>
 
 
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Revision as of 13:05, 28 July 2015

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We designed a circuit capable of producing patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger this structure formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns, providing advancements in a variety of industrial processes like for example the creation of novel bio-materials. On the other hand, this fundamental project could also speed up medical research projects on among others tumor formation and tissue regeneration.

Plasmids

“ We are not gonna buy that; We can make it “ When designing plasmids, we need to add tags and fluorescent proteins to quantify our parameters. We will also add extra restriction sites to easily generate biobricks.
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Experiments

Here you can find the following steps to become our modified organism and detailed quantification methods to determine interesting parameters.
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Results

The results of our experiments will appear here.
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