Difference between revisions of "Team:KU Leuven/Wetlab/Background"
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− | + | <p><h2>Lexicon</h2> | |
+ | Temperature sensitive cI_857= Mutated variant of the phage lambda repressor protein. It can bind firmly to the lambda promotor and represses transcription at low temperatures, but is unstable at elevated temperatures. | ||
+ | </br> | ||
+ | LuxI = enzyme for production an AHL called oxohexanoyl-homoserine lactone. This highly diffusible molecule is used as a communication molecule between cells, and is able to bind with LuxR at the lux promotor to initiate transcription. | ||
+ | </br> | ||
+ | LuxR = cytoplasmic receptor for AHL | ||
+ | </br> | ||
+ | Ag43 = membrane protein, causes autoaggregation of cells | ||
+ | </br> | ||
+ | IlvE = last enzyme in the biosynthesis of leucine. In order for it to work, we need to provide the right substrate:4-methyl-2-oxopentanoate to the medium. | ||
+ | </br> | ||
+ | Leucine = amino acid, but also a repellent if tar is knocked out | ||
+ | </br> | ||
+ | GFP = green fluorescent protein | ||
+ | </br> | ||
+ | YFP = yellow fluorescent protein | ||
+ | </br> | ||
+ | RFP = red fluorescent protein | ||
+ | </br> | ||
+ | PenI = Repressor protein, binds to the PenI regulated promotor | ||
+ | </br> | ||
+ | LVA = degradation tag, signals the linked protein for intracellular degradation | ||
+ | </br> | ||
+ | His-tag: repeat of six histidines, in order to purify it with nickelbeads | ||
+ | </br> | ||
+ | E-tag: links the tagged protein with an anti-E antibody | ||
+ | </br> | ||
+ | <br/> | ||
+ | </p> | ||
</div> | </div> | ||
</div> | </div> |
Revision as of 15:45, 28 July 2015