Difference between revisions of "Team:UCLA/Notebook/Protein Cages/31 July 2015"

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Nithin's Notes
  
 
I ran a touchdown pcr today.
 
I ran a touchdown pcr today.

Revision as of 01:35, 1 August 2015

Nithin's Notes

I ran a touchdown pcr today.

Conditions:

  • 1-cycle-
    • 98 degrees- 30s
  • 10-cycles
    • - 98 degrees- 10s
    • - 76-66 degrees (decrease by 1 degree each cycle)-30 seconds
    • - 72 degrees- 15s
  • 15-cycles
    • - 98 degrees- 10s
    • - 66 degrees- 30s
    • - 72 degrees- 15s
  • 1-cycle
    • - 72 degrees- 5 minute
    • - 4 degree hold.