Difference between revisions of "Team:UCLA/Notebook/Protein Cages/31 July 2015"
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Revision as of 01:35, 1 August 2015
Nithin's Notes
I ran a touchdown pcr today.
Conditions:
- 1-cycle-
- 98 degrees- 30s
- 10-cycles
- - 98 degrees- 10s
- - 76-66 degrees (decrease by 1 degree each cycle)-30 seconds
- - 72 degrees- 15s
- 15-cycles
- - 98 degrees- 10s
- - 66 degrees- 30s
- - 72 degrees- 15s
- 1-cycle
- - 72 degrees- 5 minute
- - 4 degree hold.