Difference between revisions of "Team:Uppsala"

Line 1: Line 1:
−
<!--<head>                      STUFF TO BE REMOVED
+
{{Uppsala_NewTemplate}}
−
 
+
−
<style>
+
−
 
+
−
body {
+
−
background-color: #ffffff;
+
−
}
+
−
 
+
−
 
+
−
#main {
+
−
background-color: #FFFFFF;
+
−
margin-left:auto;
+
−
margin-right:auto;
+
−
width:1100px;
+
−
padding: 5px;
+
−
 
+
−
}
+
−
 
+
−
h1,h2,h3 {
+
−
font-family:"Trebuchet MS", Helvetica;
+
−
color: #FF3300;
+
−
text-shadow: 2px 2px 5px #888888;
+
−
border: none;
+
−
}
+
−
 
+
−
#home_image {
+
−
  /*margin: 20px auto;*/
+
−
  display: block;
+
−
  padding: 10px 0 0 0;
+
−
}
+
−
 
+
−
hr {
+
−
background-color: #FF3300;
+
−
}
+
−
 
+
−
p {
+
−
font-family:Garamond, "Times New Roman";
+
−
font-size: 150%;
+
−
}
+
−
 
+
−
 
+
−
@import url(http://fonts.googleapis.com/css?family=Lato:300,400,700);
+
−
@charset "UTF-8";
+
−
/* Base Styles */
+
−
#cssmenu ul,
+
−
#cssmenu li,
+
−
#cssmenu a {
+
−
  list-style: none;
+
−
  margin: 0;
+
−
  padding: 0;
+
−
  border: 0;
+
−
  line-height: 1;
+
−
  font-family: 'Lato', sans-serif;
+
−
}
+
−
#cssmenu {
+
−
  -webkit-border-radius: 5px;
+
−
  -moz-border-radius: 5px;
+
−
  -ms-border-radius: 5px;
+
−
  -o-border-radius: 5px;
+
−
  border-radius: 5px;
+
−
  width: auto;
+
−
}
+
−
#cssmenu ul {
+
−
  zoom: 1;
+
−
  background: #461972;
+
−
  background: -moz-linear-gradient(top, #4619720%, #2a8a8f 100%);
+
−
  background: -webkit-gradient(linear, left top, left bottom, color-stop(0%, #461972), color-stop(100%, #2a8a8f));
+
−
  background: -webkit-linear-gradient(top, #4619720%, #2a8a8f 100%);
+
−
  background: -o-linear-gradient(top, #4619720%, #2a8a8f 100%);
+
−
  background: -ms-linear-gradient(top, #4619720%, #2a8a8f 100%);
+
−
  background: linear-gradient(top, #4619720%, #2a8a8f 100%);
+
−
  filter: progid:DXImageTransform.Microsoft.gradient(startColorstr='@top-color', endColorstr='@bottom-color', GradientType=0);
+
−
  padding: 5px 10px;
+
−
  -webkit-border-radius: 5px;
+
−
  -moz-border-radius: 5px;
+
−
  -ms-border-radius: 5px;
+
−
  -o-border-radius: 5px;
+
−
  border-radius: 5px;
+
−
}
+
−
#cssmenu ul:before {
+
−
  content: '';
+
−
  display: block;
+
−
}
+
−
#cssmenu ul:after {
+
−
  content: '';
+
−
  display: table;
+
−
  clear: both;
+
−
}
+
−
#cssmenu li {
+
−
  float: left;
+
−
  margin: 0 5px 0 0;
+
−
  border: 1px solid transparent;
+
−
}
+
−
#cssmenu li a {
+
−
  -webkit-border-radius: 5px;
+
−
  -moz-border-radius: 5px;
+
−
  -ms-border-radius: 5px;
+
−
  -o-border-radius: 5px;
+
−
  border-radius: 5px;
+
−
  padding: 8px 10px 9px 10px;
+
−
  display: block;
+
−
  text-decoration: none;
+
−
  color: #ffffff;
+
−
  border: 1px solid transparent;
+
−
  font-size: 16px;
+
−
 
+
−
}
+
−
#cssmenu li.active {
+
−
  -webkit-border-radius: 5px;
+
−
  -moz-border-radius: 5px;
+
−
  -ms-border-radius: 5px;
+
−
  -o-border-radius: 5px;
+
−
  border-radius: 5px;
+
−
  border: 1px solid #36b0b6;
+
−
}
+
−
#cssmenu li.active a {
+
−
  -webkit-border-radius: 5px;
+
−
  -moz-border-radius: 5px;
+
−
  -ms-border-radius: 5px;
+
−
  -o-border-radius: 5px;
+
−
  border-radius: 5px;
+
−
  display: block;
+
−
  background: #1e6468;
+
−
  border: 1px solid #133e40;
+
−
  -moz-box-shadow: inset 0 5px 10px #133e40;
+
−
  -webkit-box-shadow: inset 0 5px 10px #133e40;
+
−
  box-shadow: inset 0 5px 10px #133e40;
+
−
}
+
−
#cssmenu li:hover {
+
−
  -webkit-border-radius: 5px;
+
−
  -moz-border-radius: 5px;
+
−
  -ms-border-radius: 5px;
+
−
  -o-border-radius: 5px;
+
−
  border-radius: 5px;
+
−
  border: 1px solid #36b0b6;
+
−
}
+
−
#cssmenu li:hover a {
+
−
  -webkit-border-radius: 5px;
+
−
  -moz-border-radius: 5px;
+
−
  -ms-border-radius: 5px;
+
−
  -o-border-radius: 5px;
+
−
  border-radius: 5px;
+
−
  display: block;
+
−
  background: #1e6468;
+
−
  border: 1px solid #133e40;
+
−
  -moz-box-shadow: inset 0 5px 10px #133e40;
+
−
  -webkit-box-shadow: inset 0 5px 10px #133e40;
+
−
  box-shadow: inset 0 5px 10px #133e40;
+
−
}
+
−
 
+
−
 
+
−
/*Hide the sub-menus*/
+
−
nav ul ul {
+
−
display: none;
+
−
}
+
−
/*Show the sub-menus when you hover over the bar elements*/
+
−
nav ul li:hover > ul {
+
−
display: block;
+
−
}
+
−
/*Change of the box around the bar*/
+
−
nav ul {
+
−
background-color: #A5A5C1;
+
−
        background: -moz-linear-gradient(top, #efefef 0%, #bbbbbb 100%);
+
−
background: -webkit-linear-gradient(top, #efefef 0%,#bbbbbb 100%);
+
−
font-family: serif;
+
−
text-align: center;
+
−
        list-style: none;
+
−
+
−
padding: 0 15px;
+
−
display: inline-table;
+
−
}
+
−
nav > ul {
+
−
margin-top: 0px;
+
−
margin-left: auto;
+
−
margin-right: auto;
+
−
}
+
−
nav ul li {
+
−
float: left;
+
−
}
+
−
 
+
−
/*Only affects links in the first ul, the bar*/
+
−
nav ul li:hover > a{
+
−
        background-color: #4b545f;
+
−
color: #CC0000;
+
−
        text-decoration: none;
+
−
background-color: #A5A5C1;
+
−
}
+
−
 
+
−
/* Change of link text in the bar*/
+
−
nav ul li a {
+
−
display: block;
+
−
padding: 2px 15px;
+
−
text-decoration: none;
+
−
        color: white;
+
−
}
+
−
/*List of sub-menus*/
+
−
nav ul ul {
+
−
background: #5f6975;
+
−
border-radius: 0px;
+
−
padding: 0;
+
−
position: absolute;
+
−
        top: 100%;
+
−
}
+
−
/*The individual sub-menu elements*/
+
−
nav ul ul li {
+
−
float: none;
+
−
background-color: #A5A5C1;
+
−
border-top: 1px solid #6b727c;
+
−
border-bottom: 1px solid #575f6a;
+
−
position: relative;
+
−
}
+
−
/*The individual link texts in the sub-menus*/
+
−
nav ul ul li a {
+
−
padding: 15px 20px;
+
−
}
+
−
 
+
−
nav ul ul li a:hover {
+
−
background: #4b545f;
+
−
}
+
−
 
+
−
/*Supposed to make the navigation bar fixed up top*/
+
−
.fixed {
+
−
  position: fixed;
+
−
  top: 0;
+
−
  right: 0;
+
−
  left: 0;
+
−
  margin: auto;
+
−
  margin-top: 15px;
+
−
  width: 100%;
+
−
}
+
−
 
+
−
/*Div with the project buttons*/
+
−
#buttonbox {
+
−
      background-color: #d3dfe4;
+
−
      border: 2px black solid;
+
−
}
+
−
 
+
−
/*Buttons that lead to the project page*/
+
−
.buttons {
+
−
border: 3px solid black;
+
−
border-radius: 20px;
+
−
        background-color: #d3dfe4;
+
−
        margin-right: 15px;
+
−
        margin-left: 10px;
+
−
width: 355px;
+
−
height: 225px;
+
−
}
+
−
 
+
−
/*Text on the button*/
+
−
#textbox {
+
−
color: white;
+
−
padding: 5px;
+
−
background: rgba(0, 0, 0, 0.6);
+
−
display:table;
+
−
margin: 0 auto;
+
−
}
+
−
 
+
−
 
+
−
/*Reset of the wiki default settings*/
+
−
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
+
−
 
+
−
#top-section { /*-- styling for default menu bar (edit, page, history, etc.) --*/
+
−
+
−
border: 0 none;
+
−
height: 14px;
+
−
z-index: 100;
+
−
top: 0;
+
−
position: fixed;
+
−
width: 975px;
+
−
left: 50%;
+
−
margin-left: -487px;
+
−
}
+
−
 
+
−
#globalWrapper { /*-- changes default wiki settings --*/
+
−
width: 100%;
+
−
height: 100%;
+
−
border: 0px;
+
−
background-color: transparent;
+
−
margin: 0px;
+
−
padding: 0px;
+
−
}
+
−
 
+
−
#content {
+
−
background-color: #ffffff;     
+
−
width: 100%;   
+
−
border: 0px;
+
−
margin: 0px;
+
−
padding-left: 0px; /should be set to 0 in alt.1) /
+
−
padding-right: 0px;
+
−
padding-bottom: 0px;       
+
−
padding-top: 50px;
+
−
}
+
−
 
+
−
html, .wrapper { /*-- changes default wiki settings --*/
+
−
width: 100%;
+
−
height: 100%;
+
−
background-color: transparent; 
+
−
}
+
−
/*End of wiki reset*/
+
−
 
+
−
</style>
+
−
</head>
+
−
 
+
−
<body>
+
−
<div id="cssmenu">
+
−
<nav class="fixed">
+
−
<ul class="navbar">
+
−
  <li class="listelem"><a href="https://2015.igem.org/Team:Uppsala">Home</a></li>
+
−
  <li class="listelem"><a href="https://2015.igem.org/Team:Uppsala/Team">Team</a></li>
+
−
  <li class="listelem"><a href="https://2015.igem.org/Team:Uppsala/Project">Project</a>
+
−
      <ul>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Description">Description</a></li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Experiments">Experiments &amp; Protocols</a></li> 
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Results">Results</a></li> 
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Design">Design</a></li>
+
−
      </ul>
+
−
  </li>
+
−
  <li class="listelem"><a href="https://2015.igem.org/Team:Uppsala/Parts">Parts</a>
+
−
      <ul>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Parts">Team Parts</a></li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Basic_Part">Basic Parts</a></li> 
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Composite_Part">Composite Parts</a></li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Part_Collection">Part Collection</a></li> 
+
−
      </ul>
+
−
  </li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Notebook">Notebook</a></li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Attributions">Attributions</a></li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Collaborations">Collaborations</a></li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Practices">Human Practices</a></li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Safety">Safety</a></li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Modeling">Modeling</a></li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Measurement">Measurement</a></li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Software">Software</a></li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Entrepreneurship">Entrepreneurship</a></li>
+
−
</ul>
+
−
</nav>
+
−
</div>                            END OF STUFF-->
+
−
 
+
−
 
+
−
{{Template_All_Teams}}
+
 
<html>
 
<html>
−
<head>
+
<!--<head>
 
<style>
 
<style>
  
Line 526: Line 187:
 
/*End of wiki reset*/
 
/*End of wiki reset*/
  
 +
</style>
 +
-->
 +
 +
<style>
 +
 +
#home_image {
 +
  margin: 0 auto;
 +
  display: block;
 +
  margin-top: -35px
 +
}
  
 
/*Design for the buttons*/
 
/*Design for the buttons*/
Line 560: Line 231:
 
<body>
 
<body>
  
−
<nav class="fixed">
+
     <div id="main">
−
<ul id="full_width">
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala">Home</a></li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Team">Team</a></li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Description">Project</a>
+
−
      <ul>
+
−
    <li><a href="https://2015.igem.org/Team:Uppsala/Enzymes">Enzymatic Degradation<hr></a></li>
+
−
    <li><a href="https://2015.igem.org/Team:Uppsala/Biosurfactants">Biosurfactants <hr></a></li>
+
−
    <li><a href="https://2015.igem.org/Team:Uppsala/Naphthalene">Naphthalene Pathway<hr></a></li>
+
−
    <li><a href="https://2015.igem.org/Team:Uppsala/Design">Design<hr></a></li>
+
−
    <li><a href="https://2015.igem.org/Team:Uppsala/Software">Software</a></li>
+
−
      </ul>
+
−
  </li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Results">Results</a></li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Parts">Parts</a>
+
−
      <ul>
+
−
      <li><a href="https://2015.igem.org/Team:Uppsala/Basic_Part">Basic Parts<hr></a></li> 
+
−
      <li><a href="https://2015.igem.org/Team:Uppsala/Composite_Part">Composite Parts<hr></a></li>
+
−
      <li><a href="https://2015.igem.org/Team:Uppsala/Part_Collection">Part Collection</a></li> 
+
−
      </ul>
+
−
  </li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Notebook">Notebook</a>
+
−
      <ul>
+
−
        <li><a href="https://2015.igem.org/Team:Uppsala/Experiments">Experiments &amp; Protocols<hr></a></li>
+
−
        <li><a href="https://2015.igem.org/Team:Uppsala/Calendar">Calendar</a></li>
+
−
      </ul>
+
−
  </li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Attributions">Attributions</a>
+
−
     <ul>
+
−
    <li><a href="https://2015.igem.org/Team:Uppsala/Collaborations">Collaborations<hr></a></li>
+
−
    <li><a href="https://2015.igem.org/Team:Uppsala/Acknowledgements">Acknowledgements</a></li>
+
−
    </ul>
+
−
  </li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Safety">Safety</a></li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Practices">Human Practices</a></li>
+
−
  <li><a href="https://2015.igem.org/Team:Uppsala/Modeling">Modeling</a></li>
+
−
</ul>
+
−
</nav>
+
−
 
+
−
  <!--<img id="https://static.igem.org/mediawiki/2015/5/53/Ppsala_pathway.png" alt="iGEM Uppsala" style="width:1348px;height:582px;">-->
+
−
  <div id="main">
+
 
     <img id="home_image" src="https://static.igem.org/mediawiki/2015/0/08/Uppsala_NewFrontHeader.png" alt="iGEM Uppsala" style="width:1100px;height:517px;">
 
     <img id="home_image" src="https://static.igem.org/mediawiki/2015/0/08/Uppsala_NewFrontHeader.png" alt="iGEM Uppsala" style="width:1100px;height:517px;">
 
     <h1 class="header">Home</h1>
 
     <h1 class="header">Home</h1>

Revision as of 12:37, 3 August 2015

iGEM Uppsala

Home

Abstract

Nature is a biologist’s working bench - and as such we like to keep it tidy. Polycyclic aromatic hydrocarbons (PAHs) are a major pollutant derived from human activity - coil burning, oil spills, gasification plant waste. It’s carcinogenic, insoluble in water and hard to degrade. Thankfully, we’re also engineers and we decided to help nature do it’s thing.

We took a two-way approach for the project first we’ll enhance E.coli with the ability to degrade PAHs by inserting a biobrick construct containing laccase a powerful enzyme that helps get rid of those pesky compounds. And second, we’ll develop a biosensor, so that bacteria don’t waste their time producing enzymes when there is nothing to break down.

PAHs are a major problem because they are stable and hydrophobic meaning once an area is polluted, cleaning up is very hard like trying to wash a greasy pan by dipping it in cold water! By using the help of bacteria, these contaminants can be removed from the environment (for example in wastewater treatment plants). Best of all it will require minimum effort, because bacteria will degrade the compounds all by themselves, and with pleasure. Imagine eating doughnuts for homework!