Difference between revisions of "Team:Aalto-Helsinki/LabBook"

m (Week 2 table finished)
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     <td class="bottomline"></td>
−
     <td class="bottomline"><h3>May 18th - May 22nd<br>Monday</h3></td>
+
     <td class="bottomline"><h3>May 18th - May 22nd</h3></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
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     <td class="bottomline"></td>
 
     <td class="bottomline"></td>
 
     <td class="bottomline"></td>
 
     <td class="bottomline"></td>
−
     <td class="bottomline"><h3>May 18th - May 22nd<br>Monday</h3></td>
+
     <td class="bottomline"><h3>May 25th - May 29th</h3></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
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             <p>Restrict <br/>AH001-AH004 <br/> & AH006-AH008</p>
 
             <p>Restrict <br/>AH001-AH004 <br/> & AH006-AH008</p>
 
         </td>         
 
         </td>         
−
         <td class="white arrow" >
+
         <td class="white arrow">
 
             <p>Ligation</p>
 
             <p>Ligation</p>
−
         </td>      
+
         </td>          
−
        </td>       
+
 
         <td class="red arrow" >
 
         <td class="red arrow" >
−
             <p>AH002+<br/>AH004+AH008 <br/>-> AH014</p>
+
             <p>AH002+<br/>&nbsp;&nbsp;AH004+AH008 <br/>-> AH014</p>
−
         </td>      
+
         </td>    
 +
        <td class="white arrow" >
 +
            <p>Repeat ligation</p>
 +
        </td> 
 +
        <td class="blue arrow" >
 +
            <p>Restrict & <br/> extract from gel</p>
 +
        </td>   
 +
        <td class="white arrow" >
 +
            <p>Transformation <br/> into TOP10</p>
 +
        </td> 
 +
        <td class="white arrow" >
 +
            <p>Plates in rt <br/> over weekend</p>
 +
        </td> 
 
</tr>
 
</tr>
 
<tr>
 
<tr>
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         <td>
 
         <td>
 
              
 
              
 +
        </td>
 +
 +
        <td>
 +
 
         </td>
 
         </td>
 
         <td class="red arrow" >
 
         <td class="red arrow" >
−
             <p>No colonies for <br/>&nbsp;&nbsp; AH005, AH007 <br/> or AH008</p>
+
             <p>AH001+<br/> &nbsp;&nbsp; AH006+AH008 <br/>-> AH011</p>
 
         </td>
 
         </td>
 +
        <td class="white arrow" >
 +
            <p>Repeat ligation</p>
 +
        </td>
 +
        <td class="blue arrow" >
 +
            <p>Restrict & <br/> extract from gel</p>
 +
        </td>   
 +
        <td class="white arrow" >
 +
            <p>Transformation <br/> into TOP10</p>
 +
        </td> 
 +
        <td class="white arrow" >
 +
            <p>Plates in rt <br/> over weekend</p>
 +
        </td> 
 +
  </tr>
 +
 +
<tr>
 +
        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
 +
       
 +
        <td>
 +
           
 +
        </td>
 +
 +
        <td>
 +
 +
        </td>
 +
        <td class="red arrow" >
 +
            <p>AH001+<br/> &nbsp;&nbsp; AH003+AH008 <br/>-> AH013</p>
 +
        </td>
 +
        <td class="white arrow" >
 +
            <p>Repeat ligation</p>
 +
        </td>
 +
        <td class="blue arrow" >
 +
            <p>Restrict & <br/> extract from gel</p>
 +
        </td>   
 +
        <td class="white arrow" >
 +
            <p>Transformation <br/> into TOP10</p>
 +
        </td> 
 +
        <td class="white arrow" >
 +
            <p>Plates in rt <br/> over weekend</p>
 +
        </td> 
 
   </tr>
 
   </tr>
  

Revision as of 12:47, 3 August 2015

Lab Book

An example of what the table could look like:

Construct
worked with

Things done

Lab 1.1.2015
Monday

CAR

Here is my text

Here is my text

Here is my text

Here is my text

Here is my text

Here is my text

Here is my
text, there
is lot of it

another CAR?

Here is my text

Here is my text

Here is my text

Here is my text

Here is my text

Here is my text

Here is my
text, there
is lot of it

Ado?

Here is my text

Here is my text

Here is my text

Here is my text

Here is my text

Ado cont.

Here is my text

Here is my text

Here is my text

Here is my text

Here is my
text, there
is lot of it

Week 1

Construct
worked with

Things done

May 18th - May 22nd

Plates

Making Cm
plates

Stored in
+4°C

Competent Cells

Preparing
solutions for
competent cells

Stored in
+4°C

Competent Cells

o/n culture
of E. coli
TOP10

Chemical
competent
cells protocol

Tranformation
efficiency kit

Competent Cells

o/n culture
of E. coli
BL21(DE3)

Chemical
competent
cells protocol

Tranformation
efficiency kit

Colonies are
the wrong color

Propagations

Transformed
AH001-AH008
into TOP10

AH001-AH004
& AH006

Stored in
+4°C

No colonies for
   AH005, AH007
or AH008

Week 2

Construct
worked with

Things done

May 25th - May 29th

Propagations

Transformed
AH007-AH009,
AH005 & AH019
into TOP10

AH007-AH009
& AH019

o/n cultures

MiniPrep

No colonies
for AH005

Re-transform
into TOP10

Plates in rt
over weekend

Propagations

o/n culture of
AH001-AH004
& AH006

MiniPrep

Competent Cells

o/n culture
of BL21(DE3)
transformants

MiniPrep

PCR with
VF2 & VR

Smeared gels

Assembly

Restrict
AH001-AH004
& AH006-AH008

Ligation

AH002+
  AH004+AH008
-> AH014

Repeat ligation

Restrict &
extract from gel

Transformation
into TOP10

Plates in rt
over weekend

AH001+
   AH006+AH008
-> AH011

Repeat ligation

Restrict &
extract from gel

Transformation
into TOP10

Plates in rt
over weekend

AH001+
   AH003+AH008
-> AH013

Repeat ligation

Restrict &
extract from gel

Transformation
into TOP10

Plates in rt
over weekend

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 16

Week 17

Week 18