Difference between revisions of "Team:Bordeaux/Practices"
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<p align="justify"> The team who worked in the lab has received instructions of lab safety with safety training.</p> | <p align="justify"> The team who worked in the lab has received instructions of lab safety with safety training.</p> | ||
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<li align="justify"> We learned about acting in case of an accident, where to find emergency showers, fire extinguishers and emergency doors. </li> | <li align="justify"> We learned about acting in case of an accident, where to find emergency showers, fire extinguishers and emergency doors. </li> | ||
<li align="justify"> Some requirements were discussed as wearing gloves and lab coat for most procedures. In some cases, during the use of ethidium bromide, nitrile gloves are mandatory. Other experiments must be conducted with safety glasses. It is also recommended to wear clothes offering a minimum of protection. </li> | <li align="justify"> Some requirements were discussed as wearing gloves and lab coat for most procedures. In some cases, during the use of ethidium bromide, nitrile gloves are mandatory. Other experiments must be conducted with safety glasses. It is also recommended to wear clothes offering a minimum of protection. </li> | ||
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<li align="justify"> Moreover, all biological waste will be autoclaved before disposal. All work is done on benches or under open-front hoods. </li> | <li align="justify"> Moreover, all biological waste will be autoclaved before disposal. All work is done on benches or under open-front hoods. </li> | ||
<li align="justify"> We worked with non-toxic organisms as <i> Escherichia coli </i>(BL21) and <i> Saccharomyces cerevisiae </i>(Invsc1) but, all waste that has been in contact with bacteria was put to bacterial waste in aseptic conditions. </li> | <li align="justify"> We worked with non-toxic organisms as <i> Escherichia coli </i>(BL21) and <i> Saccharomyces cerevisiae </i>(Invsc1) but, all waste that has been in contact with bacteria was put to bacterial waste in aseptic conditions. </li> | ||
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<p align="justify"> On another hand, our project intends to use a polysaccharide which is synthetically produced by our genetically modified organisms but it already exists in nature. This molecule is naturally present in yeast’s wall, and doesn’t have a bad known impact on the environment. Compared to copper sulfate which is a toxic compound this molecule seems to be more eco-friendly. Indeed, this metal isn’t biodegradable; used many times to treat Downy Mildew, it is accumulated on the soil and the vineyards are polluted. For worms and micro-organisms living in vineyards soils, this compound is toxic, so it alters the biosphere. The hydric and air erosion contribute to the diffusion of copper in the transfer of pollution. </p> | <p align="justify"> On another hand, our project intends to use a polysaccharide which is synthetically produced by our genetically modified organisms but it already exists in nature. This molecule is naturally present in yeast’s wall, and doesn’t have a bad known impact on the environment. Compared to copper sulfate which is a toxic compound this molecule seems to be more eco-friendly. Indeed, this metal isn’t biodegradable; used many times to treat Downy Mildew, it is accumulated on the soil and the vineyards are polluted. For worms and micro-organisms living in vineyards soils, this compound is toxic, so it alters the biosphere. The hydric and air erosion contribute to the diffusion of copper in the transfer of pollution. </p> | ||
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<br>Three different approaches exist in synthetic biology: | <br>Three different approaches exist in synthetic biology: | ||
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<li> The metabolic engineering of the living beings by using biobricks (DNA sequences whose functions and assembly conditions are known). The biobricks are free of access on the WEB and can be synthesized on request. </li> | <li> The metabolic engineering of the living beings by using biobricks (DNA sequences whose functions and assembly conditions are known). The biobricks are free of access on the WEB and can be synthesized on request. </li> | ||
<li> The production of minimal genomes and simplified organisms where new functions can be added to realize a task. This approach is often used for the optimization of existing processes. </li> | <li> The production of minimal genomes and simplified organisms where new functions can be added to realize a task. This approach is often used for the optimization of existing processes. </li> | ||
<li> The synthesis of a whole synthetic genome that will be inserted in existing cell hosts or in synthetic cells. This field of synthetic biology may help scientists to understand how living organisms are created. </li> | <li> The synthesis of a whole synthetic genome that will be inserted in existing cell hosts or in synthetic cells. This field of synthetic biology may help scientists to understand how living organisms are created. </li> | ||
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<br>The organisms which come from these approaches can be used in both industrial applications (for example to produce drugs, biofuels, biomass or biopesticides) and in basic research as tools like biosensors (exemple) or against pollution (exemple). </p> | <br>The organisms which come from these approaches can be used in both industrial applications (for example to produce drugs, biofuels, biomass or biopesticides) and in basic research as tools like biosensors (exemple) or against pollution (exemple). </p> | ||
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<br>Among these committees we can state: | <br>Among these committees we can state: | ||
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<li> The European Commission </li> | <li> The European Commission </li> | ||
<li> The European Group on Ethics in Science and New Technologies </li> | <li> The European Group on Ethics in Science and New Technologies </li> | ||
<li> The Inter-service Group on Ethics and EU policies </li> | <li> The Inter-service Group on Ethics and EU policies </li> | ||
<li> The Executive Management for Research and Innovation </li> | <li> The Executive Management for Research and Innovation </li> | ||
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<br>Specifically for synthetic biology, a European project called SYNBIOSAFE approved by the European Commission has focused on ethics and safety of synthetic biology. In 2012, it published an <a href="http://www.biofaction.com/wp-content/uploads/2012/04/igem-biosafety-2013.pdf" target="_blank">article</a> concerning the bioethical and biosafety attentions in iGEM competitions. | <br>Specifically for synthetic biology, a European project called SYNBIOSAFE approved by the European Commission has focused on ethics and safety of synthetic biology. In 2012, it published an <a href="http://www.biofaction.com/wp-content/uploads/2012/04/igem-biosafety-2013.pdf" target="_blank">article</a> concerning the bioethical and biosafety attentions in iGEM competitions. |
Revision as of 19:59, 3 August 2015