Difference between revisions of "Team:RHIT/DesignNotebook"
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<p class="Weeks" id="Weeks"> 6/8/15-6/12/15 </p> | <p class="Weeks" id="Weeks"> 6/8/15-6/12/15 </p> | ||
<p class="Days" id="Days"> 6/8/15 </p> | <p class="Days" id="Days"> 6/8/15 </p> | ||
| − | <p class="Journal" id="Journal"></p> | + | <p class="Journal" id="Journal">Our team spent time discussing project ideas, establishing the overall goal to create an on/off “switch” for the mitochondria in<span class="italics"> S. cerevisiae</span>. We also spent time clarifying each of the medal requirements, inquiring from iGEM headquarters on questions that arose. We began discussing possibilities for parts submissions, including the ORF for<span class="italics"> mRPS12</span>, a promoter, and a reporter. Since yeast are able to survive without mitochondria on fermentable media, we will test the efficacy of our construct using a growth test on fermentable and nonfermentable carbon sources. The strains with their mitochondria turned off are expected to only grow on fermentable media. We also discussed Kozak sequences and determined that the Kozak sequence listed on the registry on the yeast page is actually the mammalian sequence. Our team began researching the consensus Kozak sequence in yeast. </p> |
<p class="Days" id="Days2"> 6/9/15 </p> | <p class="Days" id="Days2"> 6/9/15 </p> | ||
<p class="Journal" id="Journal2"></p> | <p class="Journal" id="Journal2"></p> | ||
Revision as of 15:51, 4 August 2015
Notebook
What should this page have?
- Chronological notes of what your team is doing.
- Brief descriptions of daily important events.
- Pictures of your progress.
- Mention who participated in what task.
6/8/15-6/12/15
6/8/15
Our team spent time discussing project ideas, establishing the overall goal to create an on/off “switch” for the mitochondria in S. cerevisiae. We also spent time clarifying each of the medal requirements, inquiring from iGEM headquarters on questions that arose. We began discussing possibilities for parts submissions, including the ORF for mRPS12, a promoter, and a reporter. Since yeast are able to survive without mitochondria on fermentable media, we will test the efficacy of our construct using a growth test on fermentable and nonfermentable carbon sources. The strains with their mitochondria turned off are expected to only grow on fermentable media. We also discussed Kozak sequences and determined that the Kozak sequence listed on the registry on the yeast page is actually the mammalian sequence. Our team began researching the consensus Kozak sequence in yeast.
6/9/15
6/10/15
6/11/15
6/12/15
6/15/15-6/19/15
6/15/15
6/16/15
6/17/15
6/18/15
6/19/15
6/22/15-6/26/15
6/22/15
6/23/15
6/24/15
6/25/15
6/26/15
6/29/15-7/2/15
6/29/15
6/30/15
7/1/15
7/2/15
7/6/15-7/10/15
This week was taken off by the team
7/13/15-7/17/15
7/13/15
7/14/15
7/15/15
7/16/15
7/17/15
7/20/15-7/24/15
7/20/15
7/21/15
7/22/15
7/23/15
7/24/15
6/27/15-7/31/15
7/27/15
7/28/15
7/29/15
7/30/15
7/31/15
8/3/15-8/7/15
8/3/15
8/4/15
8/5/15
8/6/15
8/7/15