Difference between revisions of "Team:Paris Saclay/Notebook/July/27"

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=Friday 27th July=
+
=Monday 27th July=
 
==Lab Work==
 
==Lab Work==
 
===New culture===
 
===New culture===

Revision as of 08:30, 5 August 2015

Monday 27th July

Lab Work

New culture

by Seong Koo

Biobricks:

  • BBa_K1707005
  • BBa_K1707006
  • BBa_K1707007

Ligation

by Pauline and Coralie

  • BBa_K1707008
    • 2µL Tp 10x
    • 1µL Ligase
    • 11µL BBa_K1707003
    • 6µL BBa_J23101
  • BBa_K1707010
    • 1µL Tp 10x
    • 1µL Ligase
    • 12µL BBa_K1399005
    • 10µL BBa_B0015
    • 1µL H20
  • BBa_K1707009
    • 2µL Tp 10x
    • 2µL Ligase
    • 10µL BBa_S03518
    • 6µL BBa_R0051
  • BBa_K1707004
    • 3µL Tp 10x
    • 2µL Ligase
    • 8µL BBa_K1707003
    • 7µL BBa_R0051


Transformation

by Pauline and Coralie

Biobricks:

  • BBa_K1707008
  • BBa_K1707010
  • BBa_K1707009
  • BBa_K1707004


Quantification

Biobricks:

  • BBa_K1707003
  • BBa_K1399005
  • BBa_B0015
  • BBa_J23101

Agarose gel 1% Migration 110V

Transformation

by Seong-Koo

Biobricks:

  • BBa_K1707005
  • BBa_K1707006
  • BBa_K1707007

Soil experiment

by Seong Koo

Plates observation and count of CFU:

  • Strain 1696:
    • Dilution 1: 424 CFU
    • Dilution 0.1: 75 CFU
    • Dilution 0.01: 1 CFU
  • Strain 1693:
    • Dilution 1: 524 CFU
    • Dilution 0.1: 35 CFU
    • Dilution 0.01: 0 CFU
  • Strain 1320:
    • Dilution 1: 111 CFU
    • Dilution 0.1: 13 CFU
    • Dilution 0.01: 0 CFU

We take 1g of contaminated soil and treat it like yesterday to spread it on specific plates Incubation ON, 37°C


Member present:

  • Instructors: Alice
  • Students: Coralie, Audrey, Pauline, Johan and Seong-Koo

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