Difference between revisions of "Team:Paris Saclay/Notebook/July/27"
| Line 49: | Line 49: | ||
| − | ===Quantification=== | + | ===Digestion + Purification + Quantification=== |
| + | '' by Audrey'' | ||
Biobricks: | Biobricks: | ||
| − | * | + | * BBa_R0051 |
| − | * | + | * BBa_K1707000 |
| − | + | ||
| − | + | ||
| − | + | Digestion: as usual, with SpeI + PstI | |
| − | + | ||
| − | === | + | Migration: Agarose gel 1%, migration 100V |
| − | ''by | + | |
| + | ===Plasmid extraction=== | ||
| + | '' by Pauline'' | ||
Biobricks: | Biobricks: | ||
| − | * | + | * BBa_K1707003 #4 et #5 |
| − | * | + | |
| − | * | + | With Plasmid DNA purification kit from Macherey-Nagel |
| + | |||
| + | ===Digestion + Purification on agarose gel=== | ||
| + | '' by Pauline'' | ||
| + | |||
| + | Biobricks: | ||
| + | * BBa_K1707003 #4 and #5 | ||
| + | |||
| + | Mix: | ||
| + | * 10µL plasmid BBa_K1707003 | ||
| + | * 1µL XbaI | ||
| + | * 1µL PstI | ||
| + | * 2µL Buffer FastDigest | ||
| + | * 6µL H2O | ||
| + | |||
| + | Purification on agarose gel 1%, migration 100V | ||
| + | Cut with a scalpel | ||
| + | Purification with the PCR Clean up kit from Macherey-Nagel | ||
| + | |||
| + | ===New Culture=== | ||
| + | ''by Johan'' | ||
| + | |||
| + | Strains: | ||
| + | * 1696 | ||
| + | * 1693 | ||
| + | * 1320 | ||
| − | + | in 150mL LB without any antibiotic | |
| − | + | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
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Revision as of 08:47, 5 August 2015
Contents
Monday 27th July
Lab Work
New culture
by Seong Koo
Biobricks:
- BBa_K1707005
- BBa_K1707006
- BBa_K1707007
Ligation
by Pauline and Coralie
- BBa_K1707008
- 2µL Tp 10x
- 1µL Ligase
- 11µL BBa_K1707003
- 6µL BBa_J23101
- BBa_K1707010
- 1µL Tp 10x
- 1µL Ligase
- 12µL BBa_K1399005
- 10µL BBa_B0015
- 1µL H20
- BBa_K1707009
- 2µL Tp 10x
- 2µL Ligase
- 10µL BBa_S03518
- 6µL BBa_R0051
- BBa_K1707004
- 3µL Tp 10x
- 2µL Ligase
- 8µL BBa_K1707003
- 7µL BBa_R0051
Transformation
by Pauline and Coralie
Biobricks:
- BBa_K1707008
- BBa_K1707010
- BBa_K1707009
- BBa_K1707004
Digestion + Purification + Quantification
by Audrey
Biobricks:
- BBa_R0051
- BBa_K1707000
Digestion: as usual, with SpeI + PstI
Migration: Agarose gel 1%, migration 100V
Plasmid extraction
by Pauline
Biobricks:
- BBa_K1707003 #4 et #5
With Plasmid DNA purification kit from Macherey-Nagel
Digestion + Purification on agarose gel
by Pauline
Biobricks:
- BBa_K1707003 #4 and #5
Mix:
- 10µL plasmid BBa_K1707003
- 1µL XbaI
- 1µL PstI
- 2µL Buffer FastDigest
- 6µL H2O
Purification on agarose gel 1%, migration 100V Cut with a scalpel Purification with the PCR Clean up kit from Macherey-Nagel
New Culture
by Johan
Strains:
- 1696
- 1693
- 1320
in 150mL LB without any antibiotic
Member present:
- Instructors: Alice
- Students: Coralie, Audrey, Pauline, Johan and Seong-Koo
Contact
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. All rights reserved All inspirations encouraged.
*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. *This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.