Difference between revisions of "Template:Team:Groningen/CONTENT/EXPERIMENTS/Grow a Biofilm on a Carrier Material"

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<div class="text">Plate 10 µL B. subtilis 3610 ComI overnight culture (OD: 2,28 (600nm) after 22h growth) on different carrier materials on LB agar and MSgg agar. MSgg was prepared according to protocol.
 
<div class="text">Plate 10 µL B. subtilis 3610 ComI overnight culture (OD: 2,28 (600nm) after 22h growth) on different carrier materials on LB agar and MSgg agar. MSgg was prepared according to protocol.
−
Carrier materials LB agar:
+
Carrier materials with LB agar as a medium:
 
</div>
 
</div>
 
<div class="listing">
 
<div class="listing">
 
<div class="item">Nylon positive charge (Boeringer, 1269299)</div>
 
<div class="item">Nylon positive charge (Boeringer, 1269299)</div>
−
<div class="item">Nytran Plus 0,45 µM (Schleicher en Schuell, AH0495-1)</div>
+
<div class="item">Nytran Plus 0.45 µm (Schleicher en Schuell, AH0495-1)</div>
 +
<div class="item">PVDF (Rocke, 03010040001)</div>
 +
<div class="item">Nitrocellulose 0.2 µm (invitrogen, LC2000)</div>
 +
<div class="item">Nylon 0.45 µm (Merck Milipore, HNWP04700)</div>
 +
<div class="item">Nitrocellulose 0.025 µm (Merck Milipore, VSWP02500)</div>
 +
<div class="item">Control (no carrier)</div>
 
</div>
 
</div>
 +
<div class="text">Carrier materials using MSgg agar as a medium:
 +
</div>
 +
<div class="listing">
 +
<div class="item">Nytran Plus 0.45 µm (Schleicher en Schuell, AH0495-1)</div>
 +
<div class="item">PVDF (Rocke, 03010040001)</div>
 +
<div class="item">Nitrocellulose 0.2µm (invitrogen, LC2000)</div>
 +
<div class="item">Nylon 0.45 µm (Merck Milipore, HNWP04700)</div>
 +
<div class="item">Nitrocellulose 0.025µm ( Merck Milipore, VSWP02500)</div>
 +
<div class="item">Control (no carrier)</div>
 +
</div>
 +
 
</html>
 
</html>
  

Revision as of 12:50, 6 August 2015

00:00, 25 March 2015 - 00:00, 26 March 2015

Plate 10 µL B. subtilis 3610 ComI overnight culture (OD: 2,28 (600nm) after 22h growth) on different carrier materials on LB agar and MSgg agar. MSgg was prepared according to protocol. Carrier materials with LB agar as a medium:
Nylon positive charge (Boeringer, 1269299)
Nytran Plus 0.45 µm (Schleicher en Schuell, AH0495-1)
PVDF (Rocke, 03010040001)
Nitrocellulose 0.2 µm (invitrogen, LC2000)
Nylon 0.45 µm (Merck Milipore, HNWP04700)
Nitrocellulose 0.025 µm (Merck Milipore, VSWP02500)
Control (no carrier)
Carrier materials using MSgg agar as a medium:
Nytran Plus 0.45 µm (Schleicher en Schuell, AH0495-1)
PVDF (Rocke, 03010040001)
Nitrocellulose 0.2µm (invitrogen, LC2000)
Nylon 0.45 µm (Merck Milipore, HNWP04700)
Nitrocellulose 0.025µm ( Merck Milipore, VSWP02500)
Control (no carrier)

Harm Ruesink
00:00, 25 March 2015 - 00:00, 26 March 2015

Plate 10 µL B. subtilis 3610 ComI overnight culture (OD: 2,28 (600nm) after 22h growth) on different carrier materials on LB agar and MSgg agar. MSgg prepared according to protocol.

Harm Ruesink