Difference between revisions of "Template:Team:Groningen/CONTENT/EXPERIMENTS/Grow a Biofilm on a Carrier Material"
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Revision as of 13:14, 6 August 2015
00:00, 25 March 2015 - 00:00, 26 March 2015
Plate 10 µL B. subtilis 3610 ComI overnight culture (OD: 2,28 (600nm) after 22h growth) on different carrier materials on LB agar and MSgg agar plates. MSgg was prepared according to protocol.
Carrier materials using LB agar as a medium
Nylon positive charge (Boeringer, 1269299)
Nytran Plus 0.45 µm (Schleicher en Schuell, AH0495-1)
PVDF (Rocke, 03010040001)
Nitrocellulose 0.2 µm (invitrogen, LC2000)
Nylon 0.45 µm (Merck Milipore, HNWP04700)
Nitrocellulose 0.025 µm (Merck Milipore, VSWP02500)
Control (no carrier)
Carrier materials using MSgg agar as a medium
Nytran Plus 0.45 µm (Schleicher en Schuell, AH0495-1)
PVDF (Rocke, 03010040001)
Nitrocellulose 0.2µm (invitrogen, LC2000)
Nylon 0.45 µm (Merck Milipore, HNWP04700)
Nitrocellulose 0.025µm ( Merck Milipore, VSWP02500)
Control (no carrier)
All plates with carrier and bacteria were put in the incubator at 30°C.
00:00, 25 March 2015 - 00:00, 26 March 2015
Plate 10 µL B. subtilis 3610 ComI overnight culture (OD: 2,28 (600nm) after 22h growth) on different carrier materials on LB agar and MSgg agar. MSgg prepared according to protocol.