Difference between revisions of "Template:Team:Groningen/CONTENT/EXPERIMENTS/Competent E. coli 2"
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|content=<div class="text">A colony was picked from a plate (±14 days old, stored in fridge) and grown overnight in liquid media (5 mL LB in 50 mL tube) in shaker incubator at 37 °C, 200 rpm</div> | |content=<div class="text">A colony was picked from a plate (±14 days old, stored in fridge) and grown overnight in liquid media (5 mL LB in 50 mL tube) in shaker incubator at 37 °C, 200 rpm</div> | ||
+ | }} | ||
+ | |||
+ | {{Team:Groningen/TEMPLATES/LOG | ||
+ | |title=00:00, 06 July 2015 - 00:00, 06 July 2015 | ||
+ | |executor=Harm Ruesink | ||
+ | |content=<div class="text"> | ||
+ | |||
+ | Tested competence of competent E. coli with Transformation efficiency kit (BBa_J04450, 50 pg/µL). Protocol: Heat Shock transformation (protocolbook).</div> | ||
+ | <div class="listing"> | ||
+ | <div class="item"> 1µL of 50pg/µL BBa_J04450 </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="text"> | ||
+ | Plate on LB agar + CM: | ||
+ | </div> | ||
+ | <div class="listing"> | ||
+ | <div class="item"> 100% (100µL) </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="text"> | ||
+ | Grow overnight in incubator at 37°C.</div> | ||
}} | }} |
Revision as of 07:42, 7 August 2015
00:00, 06 July 2015 - 00:00, 06 July 2015
A colony was picked from a plate (±14 days old, stored in fridge) and grown overnight in liquid media (5 mL LB in 50 mL tube) in shaker incubator at 37 °C, 200 rpm
00:00, 06 July 2015 - 00:00, 06 July 2015
Tested competence of competent E. coli with Transformation efficiency kit (BBa_J04450, 50 pg/µL). Protocol: Heat Shock transformation (protocolbook).
1µL of 50pg/µL BBa_J04450
Plate on LB agar + CM:
100% (100µL)
Grow overnight in incubator at 37°C.