Difference between revisions of "Team:Freiburg/Labjournals/cell free"

(Labjournal Cell-free expression)
m (Labjournal Cell-free expression)
Line 7: Line 7:
 
<!-- Labjournal content goes in here -->
 
<!-- Labjournal content goes in here -->
 
== Labjournal Cell-free expression ==
 
== Labjournal Cell-free expression ==
{{Freiburg/wiki_content_start}}
+
<html>
  * pIG15_105 PCR products were extracted from gel and purified with Qiagen. The resulting DNA showed impurities around 230 nm in repeated measurements in the NanoDrop.
+
<div class="content_background_wrapper">
 
+
<div class="central_position_wrap">
 
+
<div class="page_content">
  
 +
<p> * pIG15_105 PCR products were extracted from gel and purified with Qiagen. The resulting DNA showed impurities around 230 nm in repeated measurements in the NanoDrop.<br>
 
   -> resulting DNA was not used for expression
 
   -> resulting DNA was not used for expression
 
+
   HA-GFP-His6-His6 PCR product was retested in a control gel with a 20 µl sample. The resulting band hints at low DNA concentration <br>
 
+
   HA-GFP-His6-His6 PCR product was retested in a control gel with a 20 µl sample. The resulting band hints at low DNA concentration
+
 
   * pIG15_105 PCRs from 28.07. and 29.07. were also retested by loading 20 µl in a gel. Since again no bands showed up, the PCRs should be repeated using smaller aliquots.
 
   * pIG15_105 PCRs from 28.07. and 29.07. were also retested by loading 20 µl in a gel. Since again no bands showed up, the PCRs should be repeated using smaller aliquots.
 +
</p>
  
 
+
</div>
 
+
</div>
 
+
</div>
<!-- Labjournal content ends here -->
+
</html>
{{Freiburg/wiki_content_end}}
+

Revision as of 09:26, 7 August 2015

Labjournal Cell-free expression

* pIG15_105 PCR products were extracted from gel and purified with Qiagen. The resulting DNA showed impurities around 230 nm in repeated measurements in the NanoDrop.
-> resulting DNA was not used for expression HA-GFP-His6-His6 PCR product was retested in a control gel with a 20 µl sample. The resulting band hints at low DNA concentration
* pIG15_105 PCRs from 28.07. and 29.07. were also retested by loading 20 µl in a gel. Since again no bands showed up, the PCRs should be repeated using smaller aliquots.