Difference between revisions of "Team:Dundee/labjournal manu"
| Line 108: | Line 108: | ||
<p><b>Aim of experiment:</b> To purify <a href="http://parts.igem.org/Part:BBa_K1058008"target="_blank">BBa_K1058008</a> for sequence confirmation.</p> | <p><b>Aim of experiment:</b> To purify <a href="http://parts.igem.org/Part:BBa_K1058008"target="_blank">BBa_K1058008</a> for sequence confirmation.</p> | ||
| + | |||
| + | <p>Open link in a new window or tab: <a href="http://www.w3schools.com" target="_blank">Visit W3Schools!</a></p> | ||
<a href="#" target="_blank">Link</a> | <a href="#" target="_blank">Link</a> | ||
Revision as of 10:06, 7 August 2015
LABJOURNAL
BioSpray
Our forensic toolkit aims to use synthetic biology approaches to improve on the current methods used by crime scene investigators.
Summary
This week was all about getting started with the newly arrived biobrick BBa_K1058008
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To purify BBa_K1058008 for sequence confirmation.
Open link in a new window or tab: Visit W3Schools!
LinkProtocols Used:
Click here to see our miniprep protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Summary
This week was all about getting started with the newly arrived biobrick BBa_K1058008
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Summary
This week was all about getting started with the newly arrived biobrick BBa_K1058008
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Summary
This week was all about getting started with the newly arrived biobrick BBa_K1058008
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Summary
This week was all about getting started with the newly arrived biobrick BBa_K1058008
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Summary
This week was all about getting started with the newly arrived biobrick BBa_K1058008
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Summary
This week was all about getting started with the newly arrived biobrick BBa_K1058008
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Summary
This week was all about getting started with the newly arrived biobrick BBa_K1058008
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Summary
This week was all about getting started with the newly arrived biobrick BBa_K1058008
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Summary
This week was all about getting started with the newly arrived biobrick BBa_K1058008
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.
Protocols Used:
Click here to see our plating protocol.
Click here to see our overnight culture protocol.
Results: N/A
Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.
Restriction digests were set up according to the table below. The appropriate reaction and restriction enzymes that were used is stated in the Journal section.
|
Post Plasmid Purification |
Post PCR |
gBlock |
|
|
DNA |
1mg |
50µl |
100ng |
|
Cutsmart Buffer (µl) |
6 |
6 |
6 |
|
Water (µl) |
As required |
1 |
As required |
|
Enzyme 1 (µl) |
1.5 |
1.5 |
1.5 |
|
Enzyme 2 (µl) |
1.5 |
1.5 |
1.5 |
|
Total Volume (µl) |
30 |
60 |
30 |
The digests were incubated at 37oC for 3 hours.
Note that when performing plasmid digestions, 2.5µl of alkaline phosphatase and 2.5µl of its buffer was added at the 2 hour and 2.5 hour mark.
Ligation reactions were set up according to the table below. The vector to insert ratio is stated for each specific reaction in the Journal section.
|
Vector (µl) |
2:1 (Vector: Insert) [µl] |
3:1 (Vector: Insert) [µl] |
4:1 (Vector: Insert) [µl] |
|
|
T4 DNA Ligase |
1 |
1 |
1 |
1 |
|
Ligase Buffer |
1 |
1 |
1 |
1 |
|
Vector |
1 |
1 |
1 |
1 |
|
Insert |
- |
2 |
3 |
4 |
|
H20 |
7 |
5 |
4 |
3 |
The reactions were incubated at room temperature for at least 3 hours.
'