Difference between revisions of "Team:NCTU Formosa/Design"

Line 1: Line 1:
−
{{Team:NCTU_Formosa/project}}
+
{{Team:NCTU_Formosa/navigation}}
 +
<html>
 +
<head>
 +
<!--    匯入jQuery    -->
 +
<link href='https://2015.igem.org/Team:NCTU_Formosa/wiki_reset?action=raw&ctype=text/css' rel="stylesheet">
 +
<script type="text/javascript" src="https://2015.igem.org/Team:NCTU_Formosa/source/project_side_nav?
 +
action=raw&ctype=text/javascript"></script>
 +
<style type="text/css">
 +
body {
 +
color:#fff;
 +
margin:0;
 +
padding:0;
 +
background:#3a4044;
 +
}
 +
* {
 +
margin:0;
 +
padding:0;
 +
}
 +
/*    插入背景圖片    */
 +
    .p01{
 +
background-image:url(https://static.igem.org/mediawiki/2015/c/c0/NCTU_Formosa_metal_Style_background.jpg);
 +
        background-repeat: no-repeat;
 +
background-position:center;
 +
background-size:cover;
 +
        height:100vh;
 +
position:relative
 +
    }
 +
    .p02{
 +
        background-image:url(images/p02.jpg);
 +
        background-size:cover;
 +
        background-repeat: no-repeat;
 +
        height:100vh;
 +
    }
 +
    .p03{
 +
        background-image:url(images/p03.jpg);
 +
        background-size:cover;
 +
        background-repeat: no-repeat;
 +
        height:100vh;
 +
    }
 +
    .p04{
 +
        background-image:url(images/p04.jpg);
 +
        background-size:cover;
 +
        background-repeat: no-repeat;
 +
        height:100vh;
 +
    }
 +
    .p05{
 +
        background-image:url(images/p05.jpg);
 +
        background-size:cover;
 +
        background-repeat: no-repeat;
 +
        height:100vh;
 +
    }
 +
/*    插入導覽列    */
 +
    .sidenav{
 +
        position: fixed;
 +
        top:35vh;
 +
        right:5vw;
 +
        cursor:pointer;
 +
        z-index:3;
 +
    }
 +
    li.navli{
 +
        width:15px;
 +
        height:15px;
 +
margin: 2.5vh 0vw 0vh 0vw;
 +
        background-color: transparent;
 +
        border-radius: 10px;
 +
        list-style-type: none;
 +
border: #fff 2px solid;
 +
line-height:17px;
 +
    }
 +
    g1{
 +
        font-size:7em;
 +
        color:#fff;
 +
display:inline;
 +
    }
 +
g2{
 +
font-size:2.6em;
 +
color:#fff;
 +
display:inline;
 +
}
 +
navtext {
 +
display: block;
 +
right: 7.4vw;
 +
top: 0px;
 +
position: relative;
 +
font-weight: 100;
 +
letter-spacing: 2px;
 +
font-size: 1.9em;
 +
}
 +
.upper{
 +
position:relative;
 +
font-family: "georgia";
 +
border-bottom-style:solid;
 +
border-width:2px;
 +
height: 20vh;
 +
line-height:20vh;
 +
border-color:#fff;
 +
padding: 0vh 0vw 0vh 7vw;
 +
}
 +
.side{
 +
position:absolute;
 +
left: 0px;
 +
width: 6vw;
 +
height: 79.8vh;
 +
border-right-style:solid;
 +
border-width:2px;
 +
border-color:#fff;
 +
}
 +
.textbox{
 +
position:static;
 +
margin-left: 8vw;
 +
padding: 9vh 20vw 10vh 4vw;
 +
font-family: "Georgia";
 +
font-size:2.8em;
 +
color: #fff;
 +
}
 +
.column{
 +
height:33vh;
 +
width:100vw;
 +
text-align:center;
 +
margin:0 auto;
 +
}
 +
.line{
 +
height:0.5vh;
 +
width:100vw;
 +
background-color:#3a4044;
 +
}
 +
.head{
 +
position:absolute;
 +
top:10vh;
 +
left:40vw;
 +
height:10vh;
 +
width:20vw;
 +
margin:0 auto;
 +
font-family:zian;
 +
Color:#3a4044;
 +
font-size:5.5em;
 +
}
 +
.option{
 +
float:left;
 +
height:33vh;
 +
width:25vw;
 +
font-family:zian;
 +
Color:#3a4044;
 +
font-size:1.75em;
 +
margin:0 auto;
 +
}
 +
.TEXT{
 +
position:relative;
 +
top:25vh;
 +
width:25vw;
 +
font-family:zian;
 +
Color:#3a4044;
 +
font-size:1.75em;
 +
-webkit-transition:0.5s ease-out;
 +
-moz-transition: 0.5s ease-out;
 +
-o-transiton: 0.5s ease-out;
 +
-ms-transition:0.5s ease-out;
 +
transition:0.5s ease-out;
 +
 
 +
}
 +
.option:hover div.TEXT{
 +
-moz-transform:translateY(-23vh);
 +
-webkit-transform:translateY(-23vh);
 +
-o-transform:translateY(-23vh);
 +
-ms-transform:translateY(-23vh);
 +
transform:translateY(-23vh)
 +
}
 +
.icon{
 +
width:12%;
 +
height:24%;
 +
-webkit-transition:0.5s ease-out;
 +
-moz-transition: 0.5s ease-out;
 +
-o-transiton: 0.5s ease-out;
 +
-ms-transition:0.5s ease-out;
 +
transition:0.5s ease-out;
 +
}
 +
.option:hover div.icon{
 +
opacity:0.0;
 +
}
 +
.text2{
 +
background-color:#FFFFFF;
 +
opacity:0.0;
 +
width:17%;
 +
height:24%;
 +
-webkit-transition:0.5s ease-out;
 +
-moz-transition: 0.5s ease-out;
 +
-o-transiton: 0.5s ease-out;
 +
-ms-transition:0.5s ease-out;
 +
transition:0.5s ease-out;
 +
}
 +
.option:hover div.text2{
 +
opacity:0.2;
 +
}
 +
</style>
 +
</head>
 +
<body>
 
<div class="container">
 
<div class="container">
 
     <div class="sidenav">
 
     <div class="sidenav">
Line 11: Line 206:
 
         </ul>
 
         </ul>
 
     </div>
 
     </div>
−
     <div class="p01"><g1>PROJECT</g1></div>
+
     <div class="p01">
−
 
+
<div class="column" >
 +
<div class="head">PROJECT</div>
 +
</div>
 +
<div class="line" ></div>
 +
<div class="column" >
 +
<div class="option">
 +
<div class="icon" style="position:absolute;top:35vh;left:6vw;background-image:url();background-size:cover;"></div>
 +
<div class="text2" style="position:absolute;top:41vh;left:4vw;"></div>
 +
<div class="TEXT">Background</div>
 +
 +
</div>
 +
<div class="option">
 +
<div class="icon" style="position:absolute;top:35vh;left:31vw;background-image:url(https://static.igem.org/mediawiki/2015/3/33/2015NCTU_Formosa_design.png);background-size:cover;"></div>
 +
<div class="text2" style="position:absolute;top:41vh;left:29vw;"></div>
 +
<div class="TEXT" >Design</div>
 +
</div>
 +
<div class="option" >
 +
<div class="icon" style="position:absolute;top:35vh;left:56vw;background-image:url(https://static.igem.org/mediawiki/2015/9/9b/2015NCTU_Formosa_result.png);background-size:cover;"></div>
 +
<div class="text2" style="position:absolute;top:41vh;left:54vw;"></div>
 +
<div class="TEXT" >Result</div>
 +
</div>
 +
<div class="option">
 +
<div class="icon" style="position:absolute;top:35vh;left:81vw;background-image:url(https://static.igem.org/mediawiki/2015/8/8e/2015NCTU_Formosa_modeling.png);background-size:cover;"></div>
 +
<div class="text2" style="position:absolute;top:41vh;left:79vw;"></div>
 +
<div class="TEXT" >Modeling</div>
 +
</div>
 +
</div>
 +
<div class="line" ></div>
 +
<div class="column" ></div>
 +
</div>
 
     <div class="p02">
 
     <div class="p02">
 
     <div class="upper"><g1>Background / </g1><g2>Our project focuses on the diagnoses before and after treatment for cancers.</g2>
 
     <div class="upper"><g1>Background / </g1><g2>Our project focuses on the diagnoses before and after treatment for cancers.</g2>
Line 18: Line 242:
 
         <div class="side">
 
         <div class="side">
 
         </div>
 
         </div>
−
===1===
 
 
         <div class="textbox">Our project focuses on the diagnoses before and after treatment for cancers. Two systems are constructed for our project. The first system displays scFv on the surface of E.coli while the second one displays gold binding peptide (GBP).   
 
         <div class="textbox">Our project focuses on the diagnoses before and after treatment for cancers. Two systems are constructed for our project. The first system displays scFv on the surface of E.coli while the second one displays gold binding peptide (GBP).   
−
Before treatment, by using the fluorescent E.coli with scFv displayed on the surface, IHC procedure can be simplified because secondary antibodies is omitted and solves the problem of non-specific binding of secondary antibodies. <BR>  A dual system involving E.coli displaying scFv and GBP can solve random orientation of antibodies coating on gold chip to attain an accurate measurement. Therefore, the dual system can also offer a quantitative and accurate diagnosis for after treatment of cancer.  
+
Before treatment, by using the fluorescent E.coli with scFv displayed on the surface, IHC procedure can be simplified because secondary antibodies is omitted and solves the problem of non-specific binding of secondary antibodies.  
 +
  A dual system involving E.coli displaying scFv and GBP can solve random orientation of antibodies coating on gold chip to attain an accurate measurement. Therefore, the dual system can also offer a quantitative and accurate diagnosis for after treatment of cancer.  
 
Our project not only improves the diagnoses of the cancer types before treatment easily but also helps track the patient's condition after treatment by multifaceted measurement technology with more accurate quantitative results!
 
Our project not only improves the diagnoses of the cancer types before treatment easily but also helps track the patient's condition after treatment by multifaceted measurement technology with more accurate quantitative results!
−
 
 
         </div>
 
         </div>
 
     </div>
 
     </div>
Line 30: Line 253:
 
         <div class="side">
 
         <div class="side">
 
         </div>
 
         </div>
−
===2===
+
         <div class="textbox">ScFv is a single chain variable fragment the binding site for antigen. Comparing to the entire antibody, scFv is smaller for E.coli to display it.To display scfv on the E.coli’s surface we used a transmembrance fusion protein. This fusion protein is composed of Lipoprotein and a small part of outermembrane protein A (OmpA). Its C terminal will connect with the scFv and display it onto the membrane surface of the E.coli.
−
         <div class="textbox">scFv is a single chain variable fragment the binding site for antigen. Comparing to the entire antibody, scFv is smaller for E.coli to display it.To display scfv on the E.coli’s surface we used a transmembrance fusion protein. This fusion protein is composed of Lipoprotein and a small part of outermembrane protein A (OmpA). Its C terminal will connect with the scFv and display it onto the membrane surface of the E.coli.
+
 
         </div>
 
         </div>
 
     </div>
 
     </div>
Line 39: Line 261:
 
         <div class="side">
 
         <div class="side">
 
         </div>
 
         </div>
−
===3===
+
         <div class="textbox">testestetsetsetset
−
         <div class="textbox">
+
−
testestetsetsetset
+
 
         </div>
 
         </div>
 
     </div>
 
     </div>
Line 49: Line 269:
 
         <div class="side">
 
         <div class="side">
 
         </div>
 
         </div>
−
===4===
 
 
         <div class="textbox">testestetsetsetset
 
         <div class="textbox">testestetsetsetset
 
         </div>
 
         </div>
 
     </div>
 
     </div>
 
</div>
 
</div>
 +
 +
</body>
 +
</html>

Revision as of 04:06, 9 August 2015

  • Project
  • Background
  • Design
  • Result
  • Modeling
PROJECT
Background
Design
Result
Modeling
Background / Our project focuses on the diagnoses before and after treatment for cancers.
Our project focuses on the diagnoses before and after treatment for cancers. Two systems are constructed for our project. The first system displays scFv on the surface of E.coli while the second one displays gold binding peptide (GBP). Before treatment, by using the fluorescent E.coli with scFv displayed on the surface, IHC procedure can be simplified because secondary antibodies is omitted and solves the problem of non-specific binding of secondary antibodies. A dual system involving E.coli displaying scFv and GBP can solve random orientation of antibodies coating on gold chip to attain an accurate measurement. Therefore, the dual system can also offer a quantitative and accurate diagnosis for after treatment of cancer. Our project not only improves the diagnoses of the cancer types before treatment easily but also helps track the patient's condition after treatment by multifaceted measurement technology with more accurate quantitative results!
Design / To display scfv on the E.coli's surface we used a transmembrance fusion protein.
ScFv is a single chain variable fragment the binding site for antigen. Comparing to the entire antibody, scFv is smaller for E.coli to display it.To display scfv on the E.coli’s surface we used a transmembrance fusion protein. This fusion protein is composed of Lipoprotein and a small part of outermembrane protein A (OmpA). Its C terminal will connect with the scFv and display it onto the membrane surface of the E.coli.
Result / Sucessfully present our E.cotector
testestetsetsetset
Modeling / Find out the best condition of our E.cotector
testestetsetsetset