Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/10 August 2015"

(→‎08/10/2015)
(→‎Redid PCR to amplify M1-12 ICA product)
Line 26: Line 26:
 
| Q5
 
| Q5
 
| 0.5 uL
 
| 0.5 uL
 +
|}
 +
 +
{| class="wikitable"
 +
| 98C
 +
| 30 sec
 +
|-
 +
| 98C
 +
| 10 sec
 +
|-
 +
| 66C
 +
| 20 sec
 +
|-
 +
| 72C
 +
| 40 sec
 +
|-
 +
|
 +
| x23
 +
|-
 +
| 72C
 +
| 2 min
 +
|-
 +
| 12 C
 +
| Hold
 
|}
 
|}

Revision as of 01:51, 11 August 2015

iGEM UCLA




08/10/2015

Redid PCR to amplify M1-12 ICA product

5X Q5 buff 10 uL
10mM dNTPs 1 uL
F-03 2.5 uL
G-03 2.5 uL
Template 1 uL
ddH2O 32.5 uL
Q5 0.5 uL
98C 30 sec
98C 10 sec
66C 20 sec
72C 40 sec
x23
72C 2 min
12 C Hold