Difference between revisions of "Team:Aalto-Helsinki/Kinetics"
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<p>2\(\cdot\)Acetyl-CoA \(\rightarrow\) Acetoacetyl-CoA + CoA</p> | <p>2\(\cdot\)Acetyl-CoA \(\rightarrow\) Acetoacetyl-CoA + CoA</p> | ||
+ | <p>Vf/Vr: 22,3. Source: Molecular and catalytic properties of the acetoacetyl-coenzyme A thiolase of Escherichia coli; Archives of Biochemistry and Biophysics Volume 176, Issue 1, September 1976, Pages 159–170 | ||
+ | </p> | ||
<p>\[ \frac{K_{cat}^{AtoB} \cdot [AtoB] \cdot [Acetyl\text{-}CoA]^2}{[Acetyl\text{-}CoA]^2+2\cdot K_{M}^{AtoB:Acetyl\text{-}CoA}\cdot [Acetyl\text{-}CoA]} \]</p> | <p>\[ \frac{K_{cat}^{AtoB} \cdot [AtoB] \cdot [Acetyl\text{-}CoA]^2}{[Acetyl\text{-}CoA]^2+2\cdot K_{M}^{AtoB:Acetyl\text{-}CoA}\cdot [Acetyl\text{-}CoA]} \]</p> | ||
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<td><p>\( K_{cat}^{AtoB} \)</p></td> | <td><p>\( K_{cat}^{AtoB} \)</p></td> | ||
<td><p>10653 1/min</p></td> | <td><p>10653 1/min</p></td> | ||
− | <td | + | <td><p style="color:red">needs to be checked</p></td> |
<td><p>Forward reaction</p></td> | <td><p>Forward reaction</p></td> | ||
</tr> | </tr> | ||
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<td><p>\( K_{M}^{AtoB:Acetyl\text{-}CoA} \)</p></td> | <td><p>\( K_{M}^{AtoB:Acetyl\text{-}CoA} \)</p></td> | ||
<td><p>0.00047 mol/l</p></td> | <td><p>0.00047 mol/l</p></td> | ||
− | <td | + | <td><p>Molecular and catalytic properties of the acetoacetyl-coenzyme A thiolase of Escherichia coli; Archives of Biochemistry and Biophysics Volume 176, Issue 1, September 1976, Pages 159–170</p></td> |
<td><p>Is there something special about this?</p></td> | <td><p>Is there something special about this?</p></td> | ||
</tr> | </tr> | ||
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<td><p>\( K_{cat1}^{FadB2} \)</p></td> | <td><p>\( K_{cat1}^{FadB2} \)</p></td> | ||
<td><p>0.677 1/min</p></td> | <td><p>0.677 1/min</p></td> | ||
− | <td | + | <td><p style="color:red">needs to be checked</p></td> |
<td><p>Forward reaction</p></td> | <td><p>Forward reaction</p></td> | ||
</tr> | </tr> | ||
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<td><p>\( K_{cat2}^{FadB2} \)</p></td> | <td><p>\( K_{cat2}^{FadB2} \)</p></td> | ||
<td><p>0.723 1/min</p></td> | <td><p>0.723 1/min</p></td> | ||
− | <td | + | <td><p style="color:red">needs to be checked</p></td> |
<td><p>Reverse reaction</p></td> | <td><p>Reverse reaction</p></td> | ||
</tr> | </tr> | ||
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<td><p>\( K_{M}^{FadB2:Acetoacetyl\text{-}CoA} \)</p></td> | <td><p>\( K_{M}^{FadB2:Acetoacetyl\text{-}CoA} \)</p></td> | ||
<td><p>65.6 mol/l</p></td> | <td><p>65.6 mol/l</p></td> | ||
− | <td | + | <td><p style="color:red">needs to be checked</p></td> |
<td><p>Forward reaction</p></td> | <td><p>Forward reaction</p></td> | ||
</tr> | </tr> | ||
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<td><p>\( K_{M}^{FadB2:NADPH} \)</p></td> | <td><p>\( K_{M}^{FadB2:NADPH} \)</p></td> | ||
<td><p>50 mol/l</p></td> | <td><p>50 mol/l</p></td> | ||
− | <td | + | <td><p style="color:red">needs to be checked</p></td> |
<td><p>Forward reaction</p></td> | <td><p>Forward reaction</p></td> | ||
</tr> | </tr> | ||
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<td><p>\( K_{M}^{FadB2:3\text{-}Hydroxybutyryl\text{-}CoA} \)</p></td> | <td><p>\( K_{M}^{FadB2:3\text{-}Hydroxybutyryl\text{-}CoA} \)</p></td> | ||
<td><p>43.5 mol/l</p></td> | <td><p>43.5 mol/l</p></td> | ||
− | <td | + | <td><p style="color:red">needs to be checked</p></td> |
<td><p>Reverse reaction</p></td> | <td><p>Reverse reaction</p></td> | ||
</tr> | </tr> | ||
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<td><p>\( K_{M}^{FadB2:NADP^+} \)</p></td> | <td><p>\( K_{M}^{FadB2:NADP^+} \)</p></td> | ||
<td><p>29.5 mol/l</p></td> | <td><p>29.5 mol/l</p></td> | ||
− | <td | + | <td><p style="color:red">needs to be checked</p></td> |
<td><p>Reverse reaction</p></td> | <td><p>Reverse reaction</p></td> | ||
</tr> | </tr> | ||
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<p>Acetoacetyl-CoA + NADPH + H\(^+\) \(\rightarrow\) 3-Hydroxybutyryl-CoA + NADP\(^+\)</p> | <p>Acetoacetyl-CoA + NADPH + H\(^+\) \(\rightarrow\) 3-Hydroxybutyryl-CoA + NADP\(^+\)</p> | ||
+ | |||
+ | <p>Species is Clostridium acetobutylicum, but Clostridium Kluyveri ought to be close enough for comparison.</p> | ||
+ | |||
+ | <p>The specific activity of the 3-hydroxybutyryl-CoA dehydrogenase (forward) as measured in the direction of acetoacetyl-CoA reduction was 478.6 U/mg protein. The rate of the oxidation reaction (reverse) proceeded with 36.6 U / mg protein. | ||
+ | Source: Purification and Properties of NADP-Dependent L( +)-3-Hydroxybutyryl-CoA Dehydrogenase from Clostridiurn kluyveri; Eur. J. Biochem. 32,51-56 (1973) | ||
+ | </p> | ||
<p>\[ \frac{K_{cat}^{Hdb}\cdot [Hbd] \cdot [Acetoacetyl\text{-}CoA]\cdot [NADPH]}{[Acetoacetyl\text{-}CoA]\cdot [NADPH] + K_{M}^{Hdb:NADPH}\cdot [Acetoacetyl\text{-}CoA]+K_{M}^{Hdb:Acetoacetyl\text{-}CoA}\cdot [NADPH]} \]</p> | <p>\[ \frac{K_{cat}^{Hdb}\cdot [Hbd] \cdot [Acetoacetyl\text{-}CoA]\cdot [NADPH]}{[Acetoacetyl\text{-}CoA]\cdot [NADPH] + K_{M}^{Hdb:NADPH}\cdot [Acetoacetyl\text{-}CoA]+K_{M}^{Hdb:Acetoacetyl\text{-}CoA}\cdot [NADPH]} \]</p> | ||
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<td><p>\( K_{cat}^{Hdb} \)</p></td> | <td><p>\( K_{cat}^{Hdb} \)</p></td> | ||
<td><p>336.4 1/min</p></td> | <td><p>336.4 1/min</p></td> | ||
− | <td | + | <td><p>Purification and Properties of NADP-Dependent L( +)-3-Hydroxybutyryl-CoA Dehydrogenase from Clostridiurn kluyveri; Eur. J. Biochem. 32,51-56 (1973)</p></td> |
<td><p>Forward reaction</p></td> | <td><p>Forward reaction</p></td> | ||
</tr> | </tr> | ||
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<td><p>\( K_{M}^{Hdb:Acetoacetyl\text{-}CoA} \)</p></td> | <td><p>\( K_{M}^{Hdb:Acetoacetyl\text{-}CoA} \)</p></td> | ||
<td><p>5e-5 mol/l</p></td> | <td><p>5e-5 mol/l</p></td> | ||
− | <td | + | <td><p>Purification and Properties of NADP-Dependent L( +)-3-Hydroxybutyryl-CoA Dehydrogenase from Clostridiurn kluyveri; Eur. J. Biochem. 32,51-56 (1973)</p></td> |
<td><p>Is there something special about this?</p></td> | <td><p>Is there something special about this?</p></td> | ||
</tr> | </tr> | ||
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<td><p>\( K_{M}^{Hdb:NADPH} \)</p></td> | <td><p>\( K_{M}^{Hdb:NADPH} \)</p></td> | ||
<td><p>7e-5 mol/l</p></td> | <td><p>7e-5 mol/l</p></td> | ||
− | <td | + | <td><p>Purification and Properties of NADP-Dependent L( +)-3-Hydroxybutyryl-CoA Dehydrogenase from Clostridiurn kluyveri; Eur. J. Biochem. 32,51-56 (1973)</p></td> |
<td><p>Is there something special about this?</p></td> | <td><p>Is there something special about this?</p></td> | ||
</tr> | </tr> |
Revision as of 06:03, 11 August 2015