Difference between revisions of "Team:Aalto-Helsinki/InterLabBook"

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<ul>
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  <li><a href="https://2015.igem.org/Team:Aalto-Helsinki">Project</a>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Background">Background</a></li>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Cellulose">Cellulose</a></li>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Propane">Propane</a></li>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Micelle">Micelle</a></li>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Continuous_production">Continuous production</a></li>
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  <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Modeling">Modeling</a>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Modeling_propane">Models of propane pathway</a>
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          <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Kinetics">Kinetics of propane pathway</a></li>
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          <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Car-activation">Model of Car activation</a></li>
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        </ul>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Modeling_synergy">Synergy model</a></li>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Modeling_micelle">Micelle model</a></li>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Modeling_cellulose">About cellulose pathway</a></li>
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    </ul>
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  </li>
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  <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Lab" >Lab</a>
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    <ul>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/LabBook">Lab Book</a></li>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Protocols">Protocols</a></li>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Safety">Safety</a></li>
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  <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Results" >Results</a>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Parts">Submitted parts</a></li>
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  <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Practices">Practices</a>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Outreach">Outreach</a></li>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Questionnaire">Modeling & lab questionnaire</a></li>
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  <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Community">Community</a>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Collaborations">Collaboration</a></li>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/InterLab">Interlab study</a>
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        <ul>
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          <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/InterLabBook">InterLabBook</a></li>
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        </ul>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Software">Collab seeker</a></li>
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  <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Team">People</a>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Team">Team</a></li>
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      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Attributions">Attributions</a></li>
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    </ul>
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  </li>
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  <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Journal">Journal</a>
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  </li>
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  <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Future">Future</a>
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         <li><a href="#week5"><h4>Week 5</h4></a></li>
 
         <li><a href="#week5"><h4>Week 5</h4></a></li>
 
         <li><a href="#week6"><h4>Week 6</h4></a></li>
 
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Revision as of 13:47, 12 August 2015

Interlab Measurement Study Lab Book

Week 1

Construct
worked with

Things done

June 22nd -
June 26th

Preparations

Transform
AHD1-AHD4
into TOP10

o/n culture

MinPrep

Use iGEM's
linearized
backbones

Assembly

3A assembly
for D1-D3

Stored
in -20°C

Week 2

Construct
worked with

Things done

June 29th -
July 7th

Preparations

Transform
AHD6 & AHD7
into TOP10

o/n culture

MinPrep

Assembly

Transform
D1-D3
into TOP10

D2
expressing
GFP

Refresh
positive
colonies

No GFP

No colonies
for D1 & D3

Re-assemble
D1-D3

Transform
into TOP10

D2
successful

o/n culture

MiniPrep

No colonies
for D1 & D3

Week 3 & Week 4

Construct
worked with

Things done

July 6th -
July 10th

Checking D2

Transform D2
into TOP10

GFP
expressed

Assembly

Re-restrict
AHD1 & AHD3-AHD5

Stored
in -20°C

Week 5

Construct
worked with

Things done

July 27th -
July 31st

Assembly

Ligations
for D1 & D3

Transform
into TOP10

Checking

Check AHD1
& AHD3-AHD5
restrictions on gel

AHD4 & AHD5
OK

Too little
restriction mix
for new ligation

Re-restrict

Stored
in -20°C

Assembly

Ligations
for D1 & D3

Transform
into TOP10

AHD1 & AHD3
unclear

Re-restrict

Stored
in -20°C

Week 6

Construct
worked with

Things done

August 3rd -
August 7th

Assembly

Ligations
for D1 & D3

Transform
into TOP10

D1
expressing
GFP

o/n culture

MiniPrep

No GFP
expression
in D3

Re-restrict
AHD3-AHD5

Ligate &
transform
into TOP10

No GFP
expression

Checking

Mistake in AHD4
restriction
protocol

Check AHD4
restriction
on gel

Restriction OK

Due to the limited time we had to use for our project, we had to abort the interlab measurement study. We were able to construct two devices and measure the GFP from these. We were unable to build the device with J23106 promoter.