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Construct |
Things done |
June 22nd - |
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Preparations |
Transform |
o/n culture |
MinPrep |
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|
Use iGEM's |
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Assembly |
3A assembly |
Stored |
Construct |
Things done |
June 29th - |
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Preparations |
Transform |
o/n culture |
MinPrep |
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Assembly |
Transform |
D2 |
Refresh |
No GFP |
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|
No colonies |
Re-assemble |
Transform |
D2 |
o/n culture |
MiniPrep |
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|
No colonies |
Construct |
Things done |
July 6th - |
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Checking D2 |
Transform D2 |
GFP |
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Assembly |
Re-restrict |
Stored |
Construct |
Things done |
July 27th - |
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Assembly |
Ligations |
Transform |
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Checking |
Check AHD1 |
AHD4 & AHD5 |
Too little |
Re-restrict |
Stored |
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Assembly |
Ligations |
Transform |
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|
AHD1 & AHD3 |
Re-restrict |
Stored |
Construct |
Things done |
August 3rd - |
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Assembly |
Ligations |
Transform |
D1 |
o/n culture |
MiniPrep |
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|
No GFP |
Re-restrict |
Ligate & |
No GFP |
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Checking |
Mistake in AHD4 |
Check AHD4 |
Restriction OK |
Due to the limited time we had to use for our project, we had to abort the interlab measurement study. We were able to construct two devices and measure the GFP from these. We were unable to build the device with J23106 promoter.