Difference between revisions of "Team:Technion Israel/Lab Notebook/Overview"
| Line 157: | Line 157: | ||
<div class="ecoli"> | <div class="ecoli"> | ||
<h2>E.coli</h2> | <h2>E.coli</h2> | ||
| − | <p> | + | <p>Planned the Gibson reaction for our gBlock of 3α-HSD and the plasmid N155 (from our mentors) containing pT7, RBS and mcherry. |
| + | </p> | ||
| + | <p>Ordered the gBlock of 3α-HSD, which was optimized for E. coli codon usage. We also ordered primers for the Gibson reaction for the gBlock.</p> | ||
| + | <p>Performed reverse PCR to the N155 plasmid and carried out a DPN1 reaction on the product.</p> | ||
| + | <p></p><p><i>Active team members this week: Adi and Alexey</i></p> | ||
| + | |||
</div> | </div> | ||
<div class="modeling"> | <div class="modeling"> | ||
Revision as of 12:57, 13 August 2015
Lab Notebook Overview
Week 1
Bacillus
content
E.coli
Planned the Gibson reaction for our gBlock of 3α-HSD and the plasmid N155 (from our mentors) containing pT7, RBS and mcherry.
Ordered the gBlock of 3α-HSD, which was optimized for E. coli codon usage. We also ordered primers for the Gibson reaction for the gBlock.
Performed reverse PCR to the N155 plasmid and carried out a DPN1 reaction on the product.
Active team members this week: Adi and Alexey
Modeling
content
Comb
content
NADPH
content
Week 2
Bacillus
content
E.coli
content
Modeling
content
Comb
content
NADPH
content