Difference between revisions of "Team:Technion Israel/Lab Notebook/Overview"
| Line 149: | Line 149: | ||
<div class="notebook_wrapper"> | <div class="notebook_wrapper"> | ||
<div class="week"> | <div class="week"> | ||
| − | <h1>Week 1</h1> | + | <h1>Week 1: May 2-8</h1> |
<div class="week_content"> | <div class="week_content"> | ||
<div class="bacillus"> | <div class="bacillus"> | ||
| Line 157: | Line 157: | ||
<div class="ecoli"> | <div class="ecoli"> | ||
<h2>E.coli</h2> | <h2>E.coli</h2> | ||
| − | <p>Planned the Gibson reaction for our gBlock of 3α-HSD and the plasmid N155 (from our mentors) containing pT7, RBS and mcherry. | + | <p>Planned the Gibson reaction for our gBlock of 3α-HSD and the plasmid N155 (from our mentors) containing pT7, RBS and mcherry.</p> |
| − | </p> | + | <p>Ordered the gBlock of 3α-HSD, which was optimized for E. coli codon usage. We also ordered primers for the Gibson reaction for the gBlock.</p> |
| − | <p>Ordered the gBlock of 3α-HSD, which was optimized for E. coli codon usage. We also ordered primers for the Gibson reaction for the gBlock.</p> | + | <p>Performed reverse PCR to the N155 plasmid and carried out a DPN1 reaction on the product.</p> |
| − | <p>Performed reverse PCR to the N155 plasmid and carried out a DPN1 reaction on the product.</p> | + | <p></p><p><i>Active team members this week: Adi and Alexey</i></p> |
| − | <p></p><p><i>Active team members this week: Adi and Alexey</i></p> | + | |
</div> | </div> | ||
| Line 179: | Line 178: | ||
</div> | </div> | ||
<div class="week"> | <div class="week"> | ||
| − | <h1>Week 2</h1> | + | <h1>Week 2: May 9-15</h1> |
<div class="week_content"> | <div class="week_content"> | ||
<div class="bacillus"> | <div class="bacillus"> | ||
| Line 187: | Line 186: | ||
<div class="ecoli"> | <div class="ecoli"> | ||
<h2>E.coli</h2> | <h2>E.coli</h2> | ||
| + | <p>Our gBlock arrived!!!</p> | ||
| + | <p>We performed PCR on the 3αHSD gBlock with only 10 cycles (as recommended), but received no band in the gel</p> | ||
| + | <p>We performed PCR on the 3αHSD gBlock in higher temperatures, but still received no bands in the gel</p> | ||
| + | <p></p><p><i>Active team members this week: Adi</i></p> | ||
| + | |||
| + | </div> | ||
| + | <div class="modeling"> | ||
| + | <h2>Modeling</h2> | ||
<p>content</p> | <p>content</p> | ||
| + | </div> | ||
| + | <div class="comb"> | ||
| + | <h2>Comb</h2> | ||
| + | <p>content</p> | ||
| + | </div> | ||
| + | <div class="nadph"> | ||
| + | <h2>NADPH</h2> | ||
| + | <p>content</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="week"> | ||
| + | <h1>Week 3: May 16-22</h1> | ||
| + | <div class="week_content"> | ||
| + | <div class="bacillus"> | ||
| + | <h2>Bacillus</h2> | ||
| + | <p>content</p> | ||
| + | </div> | ||
| + | <div class="ecoli"> | ||
| + | <h2>E.coli</h2> | ||
| + | <p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p> | ||
| + | <p></p><p><i>Active team members this week: Alexey</i></p> | ||
| + | |||
</div> | </div> | ||
<div class="modeling"> | <div class="modeling"> | ||
Revision as of 15:08, 13 August 2015
Lab Notebook Overview
Week 1: May 2-8
Bacillus
content
E.coli
Planned the Gibson reaction for our gBlock of 3α-HSD and the plasmid N155 (from our mentors) containing pT7, RBS and mcherry.
Ordered the gBlock of 3α-HSD, which was optimized for E. coli codon usage. We also ordered primers for the Gibson reaction for the gBlock.
Performed reverse PCR to the N155 plasmid and carried out a DPN1 reaction on the product.
Active team members this week: Adi and Alexey
Modeling
content
Comb
content
NADPH
content
Week 2: May 9-15
Bacillus
content
E.coli
Our gBlock arrived!!!
We performed PCR on the 3αHSD gBlock with only 10 cycles (as recommended), but received no band in the gel
We performed PCR on the 3αHSD gBlock in higher temperatures, but still received no bands in the gel
Active team members this week: Adi
Modeling
content
Comb
content
NADPH
content
Week 3: May 16-22
Bacillus
content
E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
content
Comb
content
NADPH
content