Difference between revisions of "Team:UiOslo Norway/Experiments/Alkaline Phospatase"

(Created page with "{{UiOslo_Norway}} <html> <h1>Shrimp Alkaline Phosphatase Treatment (Fermentas):</h1> <a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments" > Back to Protocols </...")
 
Line 10: Line 10:
 
Back to Protocols
 
Back to Protocols
 
</a>  
 
</a>  
 +
<p></br></p>
 +
 +
<p>
 +
Use Zero blunt TOPO PCR cloning Kit</br>
 +
</br>
 +
<p>
 +
2 μl 10X Reaction Buffer</br>
 +
1 μl shrimp alkaline phosphatase</br>
 +
x μl DNA( 1 μg total concentration)</br>
 +
MilliQ up to 20 μl</br>
 +
</p>
 +
<hr style="height:2px;border:none;color:#472400;background-color:#333;" />
 +
<p>Total Reaction of 20 μl</p>
 +
 +
</br></p>
 +
<p>
 +
Incubate at 37 °C for 30 minutes
 +
Heatinactivation at 65 °C for 15 minutes.
 +
</p>
  
 
</div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
 
</div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
 
</html>
 
</html>

Revision as of 08:11, 14 August 2015

Shrimp Alkaline Phosphatase Treatment (Fermentas):

Back to Protocols


Use Zero blunt TOPO PCR cloning Kit

2 μl 10X Reaction Buffer
1 μl shrimp alkaline phosphatase
x μl DNA( 1 μg total concentration)
MilliQ up to 20 μl

Total Reaction of 20 μl


Incubate at 37 °C for 30 minutes Heatinactivation at 65 °C for 15 minutes.