Difference between revisions of "Team:UiOslo Norway/Experiments/PCR taq"
| Line 24: | Line 24: | ||
0.125 µl Taq DNA Polymerase</br> | 0.125 µl Taq DNA Polymerase</br> | ||
17.875 µl MilliQ Water </br> | 17.875 µl MilliQ Water </br> | ||
| − | |||
<hr style="height:2px;border:none;color:#472400;background-color:#333;" /> | <hr style="height:2px;border:none;color:#472400;background-color:#333;" /> | ||
| − | + | 25 µl Total Reaction </li> | |
| − | </li> | + | |
</br> | </br> | ||
| − | < | + | <ol> |
| + | <li><p><b>PCR Program:</b></br> | ||
| + | |||
| + | </li> | ||
</div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.--> | </div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.--> | ||
</html> | </html> | ||
Revision as of 08:30, 14 August 2015
PCR with Taq DNA Polymerase (NEB):
Back to Protocols
Reaction Setup: 2.5 µl 10X Standard Taq Reaction buffer 2.5 µl dNTPS [2 mM] 0.5 µl Forward Primer [10 µM] 0.5 µl Reverse Primer [10 µM] 1 µl Template DNA 0.125 µl Taq DNA Polymerase 17.875 µl MilliQ Water
25 µl Total ReactionPCR Program: