Difference between revisions of "Team:UiOslo Norway/Experiments/PCR taq"
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17.875 µl MilliQ Water </br> | 17.875 µl MilliQ Water </br> | ||
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| − | 25 µl Total Reaction </li> | + | <p>25 µl Total Reaction </p></li> |
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<li><p><b>PCR Program:</b></br> | <li><p><b>PCR Program:</b></br> | ||
Revision as of 08:33, 14 August 2015
PCR with Taq DNA Polymerase (NEB):
Back to Protocols
Reaction Setup: 2.5 µl 10X Standard Taq Reaction buffer 2.5 µl dNTPS [2 mM] 0.5 µl Forward Primer [10 µM] 0.5 µl Reverse Primer [10 µM] 1 µl Template DNA 0.125 µl Taq DNA Polymerase 17.875 µl MilliQ Water
25 µl Total Reaction
PCR Program: