User contributions
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- 02:56, 14 August 2015 (diff | hist) . . (+941) . . N Team:UCLA/Notebook/Protein Cages/13 August 2015 (Created page with "{{Template:UCLA}} Phillip's notes: Introduction: The PCquad Yeates will be grown again in order to rerun the purification and a purer product will be attempted to be obtained....") (current)
- 02:15, 13 August 2015 (diff | hist) . . (+1,467) . . N Team:UCLA/Notebook/Protein Cages/11 August 2015 (Created page with "{{Template:UCLA}} Phillip's notes: Introduction: An SDS-PAGE gel will be ran to analyze the results of yesterday’s purification. Procedures: All samples were obtained a...")
- 02:14, 13 August 2015 (diff | hist) . . (0) . . N File:2015-08-12 SDSPAGE Yeates PCquad.jpg (current)
- 02:00, 13 August 2015 (diff | hist) . . (+3,928) . . Team:UCLA/Notebook/Protein Cages/10 August 2015
- 01:22, 8 August 2015 (diff | hist) . . (+582) . . N Team:UCLA/Notebook/Protein Cages/7 August 2015 (Created page with "{{Template:UCLA}} Introduction: Today gel extraction and digestion of PCquad 3.0 will be done. If permitting, ligation will also be done. Procedures: Gel extraction was perfo...")
- 17:51, 5 August 2015 (diff | hist) . . (+8) . . Team:UCLA/Notebook/Protein Cages/4 August 2015 (current)
- 02:01, 5 August 2015 (diff | hist) . . (+2,263) . . N Team:UCLA/Notebook/Protein Cages/4 August 2015 (Created page with "{{Template:UCLA}} Phillip's notes: Introduction: The gBlocks and primers have all arrived. They will be resuspended, and the initial amplification will be done. Procedures: ...")
- 01:56, 5 August 2015 (diff | hist) . . (0) . . N File:2015-08-04 PCR PCquad mutant 10 amplification 61C, 65C, 69C.JPG (current)
- 01:56, 5 August 2015 (diff | hist) . . (0) . . N File:2015-08-04 PCR PCquad 3.0 amplification 61C, 65C, 69C.JPG (current)
- 18:33, 30 July 2015 (diff | hist) . . (+2,121) . . N Team:UCLA/Notebook/Protein Cages/29 July 2015 (Created page with "{{Template:UCLA}} Phillip's notes: Introduction: It has been decided that a new gBlock will be ordered. After some consultation, it has been decided that the gBlock will be d...") (current)
- 02:04, 28 July 2015 (diff | hist) . . (+1,700) . . Team:UCLA/Notebook/Protein Cages/27 July 2015 (current)
- 02:01, 28 July 2015 (diff | hist) . . (0) . . N File:2015-07-27 PCR PCquad amplification pET22b 64, 68, 72.JPG (current)
- 02:07, 22 July 2015 (diff | hist) . . (+1,545) . . Team:UCLA/Notebook/Protein Cages/21 July 2015 (current)
- 02:11, 21 July 2015 (diff | hist) . . (+1,826) . . N Team:UCLA/Notebook/Protein Cages/20 July 2015 (Created page with "{{Template:UCLA}} Phillip's notes: Introduction: PCR will be repeated again to amplify our construct. A longer extension time will be used, as well as running only the prim...")
- 23:29, 17 July 2015 (diff | hist) . . (+385) . . Team:UCLA/Notebook/Protein Cages/17 July 2015 (current)
- 23:25, 17 July 2015 (diff | hist) . . (+1) . . Team:UCLA/Notebook/Protein Cages/17 July 2015
- 23:22, 17 July 2015 (diff | hist) . . (+76) . . Team:UCLA/Notebook/Protein Cages/17 July 2015
- 23:20, 17 July 2015 (diff | hist) . . (0) . . N File:2015-07-17 PCR PCquad amplification 62C, 66C, 70C .JPG (current)
- 22:49, 17 July 2015 (diff | hist) . . (+7,089) . . Team:UCLA/Notebook/Protein Cages/17 July 2015
- 19:08, 17 July 2015 (diff | hist) . . (+471) . . Team:UCLA/Notebook/Protein Cages/14 July 2015 (current)
- 19:07, 17 July 2015 (diff | hist) . . (+978) . . Team:UCLA/Notebook/Protein Cages/13 July 2015 (current)
- 19:06, 17 July 2015 (diff | hist) . . (-1,456) . . Team:UCLA/Notebook/Protein Cages/17 July 2015 (Replaced content with "{{Template:UCLA}}")
- 19:05, 17 July 2015 (diff | hist) . . (+1,473) . . N Team:UCLA/Notebook/Protein Cages/17 July 2015 (Created page with "{{Template:UCLA}} Phillip's notes Introduction: Cells will be harvested, and cell lysis will be done if reagents are available. Procedures: Harvesting of cells The 2L fla...")
- 02:10, 16 July 2015 (diff | hist) . . (+986) . . Team:UCLA/Notebook/Protein Cages/14 July 2015
- 02:09, 16 July 2015 (diff | hist) . . (+20) . . Team:UCLA/Notebook/Protein Cages/12 July 2015 (current)
- 02:01, 16 July 2015 (diff | hist) . . (+71) . . Team:UCLA/Notebook/Protein Cages/12 July 2015
- 02:00, 16 July 2015 (diff | hist) . . (+399) . . N Team:UCLA/Notebook/Protein Cages/12 July 2015 (Created page with "{{Template:UCLA}} Introduction: Starter culture will be done today. Procedures: 10mL of LB was added to a culture tube. For a final concentration of 100ug/mL of carbenicilli...")
- 01:02, 16 July 2015 (diff | hist) . . (+954) . . N Team:UCLA/Notebook/Protein Cages/11 July 2015 (Created page with "{{Template:UCLA}} Phillip's Notes Introduction: Today begins the first attempt at expressing the protein cage received from Yeates. The glycerol stock will be used to streak ...") (current)
- 00:39, 10 July 2015 (diff | hist) . . (+10) . . Team:UCLA/Notebook/Protein Cages (→Achievements)
- 00:38, 10 July 2015 (diff | hist) . . (+20) . . Team:UCLA/Notebook/Protein Cages/9 July 2015
- 00:33, 10 July 2015 (diff | hist) . . (+5,258) . . N Team:UCLA/Notebook/Protein Cages/9 July 2015 (Created page with "{{Template:UCLA}} Introduction: Today ampicillin plates will be made in order to have them available for protein expression. In addition, the PCquad optimized will be designed...")
- 02:49, 9 July 2015 (diff | hist) . . (+8,105) . . N Team:UCLA/Notebook/Protein Cages/8 July 2015 (Created page with "{{Template:UCLA}} Phillip's notes: Introduction: For today, colony PCR will be done in order to verify the Yeate construct. In addition, ampicillin plates will be made in pre...") (current)
- 00:29, 7 July 2015 (diff | hist) . . (+1,487) . . N Team:UCLA/Notebook/Protein Cages/6 July 2015 (Created page with "{{Template:UCLA}} Phillip's notes: Introduction: For today, colony PCR primers will be designed in order to use them to verify that the correct plasmid and gene is inside our ...") (current)
- 00:12, 3 July 2015 (diff | hist) . . (+728) . . Team:UCLA/Notebook/Protein Cages/2 July 2015 (current)
- 19:39, 2 July 2015 (diff | hist) . . (+6,108) . . Team:UCLA/Notebook/Protein Cages/2 July 2015
- 23:01, 1 July 2015 (diff | hist) . . (+14) . . Team:UCLA/Notebook/Protein Cages/1 July 2015
- 23:00, 1 July 2015 (diff | hist) . . (+857) . . N Team:UCLA/Notebook/Protein Cages/1 July 2015 (Created page with "{{Template:UCLA}} Phillip's notes: Introduction: Today the project overview will be discussed during our general meeting with Dr. Kosuri. Fasih will then help us take our gly...")
- 22:29, 30 June 2015 (diff | hist) . . (+2,966) . . N Team:UCLA/Notebook/Protein Cages/30 June 2015 (Created page with "{{Template:UCLA}} Phillip's notes: Introduction: Today, a meeting will be held with David in order to check our designed primers for PCquad for pET22b as well as how to desi...") (current)
- 02:19, 30 June 2015 (diff | hist) . . (+2,324) . . Team:UCLA/Notebook/Protein Cages/29 June 2015
- 19:10, 29 June 2015 (diff | hist) . . (+1,190) . . Team:UCLA/Notebook/Protein Cages/26 June 2015
- 01:34, 26 June 2015 (diff | hist) . . (+2,899) . . Team:UCLA/Notebook/Protein Cages/25 June 2015
- 00:52, 25 June 2015 (diff | hist) . . (+3,762) . . N Team:UCLA/Notebook/Protein Cages/24 June 2015 (Created page with "{{Template:UCLA}} Phillip's notes Introduction: Today will be focused on designing the complete constructs to be ordered. The sequence from Yen will be used. They will be de...")
- 20:59, 24 June 2015 (diff | hist) . . (0) . . Team:UCLA/Notebook/Protein Cages
- 01:17, 24 June 2015 (diff | hist) . . (+3,859) . . Team:UCLA/Notebook/Protein Cages/23 June 2015
- 23:51, 2 June 2015 (diff | hist) . . (+417) . . N Team:UCLA/Notebook/Protein Cages/2 July 2015 (Created page with "{{Template:UCLA}} Notes by Phillip The sequence of mutant cages were designed on benchling. Using http://www.bioinformatics.org/sms2/rev_trans.html The insertion sequence for mu...")
- 23:10, 2 June 2015 (diff | hist) . . (+1) . . Team:UCLA/Notebook/Protein Cages (→Design)
- 00:56, 27 May 2015 (diff | hist) . . (+576) . . Team:UCLA/Notebook/Protein Cages/26 May 2015 (current)
- 00:51, 27 May 2015 (diff | hist) . . (-6) . . Team:UCLA/Notebook/Protein Cages (→Design)
- 00:49, 27 May 2015 (diff | hist) . . (+1) . . Team:UCLA/Notebook/Protein Cages (→Design)
- 00:47, 27 May 2015 (diff | hist) . . (+21) . . Team:UCLA/Notebook/Protein Cages (→Design)
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