Team:UiOslo Norway/Experiments/SDS-Page

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SDS-Page:

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• Resuspend cell pellet in 600 ul PBS buffer (pH 7.4)

• Add glass beads and disrupt cells by vortexing

• Centrifuge at 16.000 x g for 5 minutes

• Transfer soluble fraction into a new tube and resuspend insoluble fraction in 600 ul PBS buffer (pH 7.4)