Team:UiOslo Norway/Experiments/SDS-Page

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SDS-Page:

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Reagent

1. Stacking Gel:
   
   0.7 ml 30 % Acrylamide (37.5:1)
   1.25 ml Stacking gel buffer (1 M Tris pH 6.8)
   3 ml Water
   50 ul 10 % (w/v) SDS
   5 ul TEMED
   50 ul APS
   




2. Separating gel:
   
   12 % 18 %
   

   ---

   2 ml 2 ml Seperating buffer (1.5 M Tris pH 8.8)
   3.2 ml 4.8 ml 30 % Acrylamide (37.5:1)
   2.7 ml 1.1 ml Water
   80 ul 80 ul 10% (w/v) SDS
   8 ul 8 ul TEMED
   80 ul 80 ul APS
   
   Run SDS-Page for 1 hour at 30 mA per gel

Coomassie-staining

Quick Coomassie staining solution:

1 % (w/v) Coomassie Brilliant Blue R250
50 % (v/v) Methanol

10 % (v/v) Acetic acid
Coomassie Destaining solution:
40 % (v/v) Methanol

10 % (v/v) Acetic acid