Team:Paris Bettencourt/jb
o15.053 | HO-Poly-KanMX4-HO amplification (reverse) + tail | TGAAATGGCGAGTATTGATAATGATAAACTCTGTCAAGGAGGGTATTCTGGGC |
o15.056 | gBlock2 amplification (forward) + tail | TCACAACAAGAATTTTGCAGAGGACCTAACCGAAGGAAAGTTTTCCTTCCCAACAATC |
o15.057 | gBlock2 amplification (reverse) + tail | GGACTTGATAGAATTTTGTCTGGTTGACTCGGATGCAATGGAGGACCGAACAAT |
o15.058 | gBlock3 amplification (forward) | AACGTTATTGTTCGGTCCTCCATTGC |
o15.059 | gBlock3 amplification (reverse) + tail | GTCCAGATTGACTCGAATGGGTGTAATGCCAGTATTTGACCAATGAATTGTAGCCTCAAG |
o15.060 | gBlock4 amplification (forward) | CTTGAGGCTACAATTCATTGGTCAAATAC |
o15.061 | gBlock4 amplification (reverse) + tail | TGTACGGGCGACAGTCACATCATGCCCCTGCAGGGTACCGCGAATTCCCC |
o15.117 | forward primer for gblock 1 part 1 | CGTCCAAGACATACTGCGTTTACG |
o15.118 | reverse primer for gblock 1 part 1 | GTGTGCAACATTCCCACTACATTTTGAATG |
o15.119 | forward primer for gblock 1 part 2 | CATTCAAAATGTAGTGGGAATGTTGCAC |
o15.120 | reverse primer for gblock 1 part 2 | TGGATTGTTGGGAAGGAAAACTTTCCTT |
o15.121 | forward primer gblock 1 + restriction site ecor1 | TATACTGCAGTCTAGAACTAGTGGATCCATGGATTAC |
o15.122 | reverse primer gblock 1 + tail | AGCCTGGAGGAAGGGTTAGCATGGATGGAATGGATTGTTGGGAAGGAAAACTTTCCTT |
o15.123 | forward primer TDH3 from registry with HO binding tail | GCCCAGAATACCCTCCTTGACAGACAGTTTATTCCTGGCATCCACTAAATATAATG |
o15.124 | reverse primer TDH3 from registry with RE binding site | TATACTGCAGTTTGTTTGTTTATGTGTGTTTATTCGAAACTAAGTTC |
o15.125 | forward primer full gblock 1 with new promoter | GCCCAGAATACCCTCCTTGACAG |
o15.126 | reverse primer full gblock 1 with new promoter | CATGCTAACCCTTCCTCCAGGCT |
o15.127 | forward primer gblock 2 + tail | TCACAACAAGAATTTTGCAGAGGACCTAACCGAAGGAAAGTTTTCCTTCCCAACAATC |
o15.128 | reverse primer gblock 2 + tail | GGACTTGATAGAATTTTGTCTGGTTGACTCGGATGCAATGGAGGACCGAACAAT |
o15.129 | forward primer gblock 3 | AACGTTATTGTTCGGTCCTCCATTGC |
o15.130 | reverse primer gblock 3 + tail | GTCCAGATTGACTCGAATGGGTGTAATGCCAGTATTTGACCAATGAATTGTAGCCTCAAG |
o15.131 | forward primer gblock 4 | CTTGAGGCTACAATTCATTGGTCAAATAC |
o15.132 | reverse primer gblock 4 HO tail | TGTACGGGCGACAGTCACATCATGCCCCTGCAGGGTACCGCGAATTCCCC |
o15.133 | reverse primer gblock 4 HMG tail | CATGCGCCTATTATTACTTCAATAGATGGCAGGGTACCGCGAATTCCCC |
o15.134 | forward primer vector HO with HMG | GCTTTAATTTGCGGCCGGTACCCAATTCGCCAGGGGCATGATGTGACTGTCG |
o15.135 | forward primer vector HO without HMG (binds to gBlock4) | TCGCTATACTGGGGAATTCGCGGTACCCTGCAGGGGCATGATGTGACTGTCG |
o15.136 | reverse primer vector HO (binds to TDH3?) | TATATTTAGTGGATGCCAGGAATAAACTGTCTGTCAAGGAGGGTATTCTGGGC |
o15.137 | formard primer HMG+tail with RE | TATATCTAGACCCGCCGCCACCATGCAAAG |
o15.138 | reverse primer HMG+tail with restriction site | TATACTCGAGTCACTGTTGAGACCTCAAATCCTGTAAG |
o15.139 | forward primer promoter HMG terminator | TCGCTATACTGGGGAATTCGCGGTACCCTGCCATCTATTGAAGTAATAATAGGCGCATG |
o15.140 | reverse primer HMG + tail HO | TGTACGGGCGACAGTCACATCATGCCCCTGGCGAATTGGGTACCGGCCG |
o15.141 | reverse primer gblock 1 part 1 (binds to part2)) | TCCTCAACGTCGTCCATCAGTAAGCTCGCTGTGTGCAACATTCCCACTACATTTTGAATG |
o15.142 | forward primer gblock 1 + tail that binds to HO pl | GCCCAGAATACCCTCCTTGACAGCGTCCAAGACATACTGCGTTTACG |
o15.143 | reverse primer HO + tail crappy ADH1 | TCGACACGTAAACGCAGTATGTCTTGGACGCTGTCAAGGAGGGTATTCTGGGC |
o15.144 | forward primer gblock 1 + tail TDH3 | GTTTCGAATAAACACACATAAACAAACAAATCTAGAACTAGTGGATCCATGGATTAC |
o15.145 | reverse primer TDH3 + tail for kozac | TCCATGGATCCACTAGTTCTAGATTTGTTTGTTTATGTGTGTTTATTCGAAACTAAGTTC |