Team:USTC
Project
NDM: Nanomachine Detecting Microbiotics
Abusing antibiotics has caused severe antibiotics contamination and resistance issues worldwide. Therefore, we USTC develop a device NDM with OD detector and optical interference path, recognition program based on RasberryPi and Arduino to detect antibiotics in natural water bodies. In NDM, there are two bacterial systems for measurement: ROSE, an engineered bacterial reporter system integrated with permeability modification, logic amplification circuit and quorum sensing, is able to adjust EGFP expression level, and NDM reads fluorescence intensity from ROSE to send results in high resolution. CACCI, chemotaxis and transmembrane protein-modified bacteria, are chemically covalently adhered to a polymer membrane. Deformation caused by CACCI motility can be recognized with optical interference and the interference pattern recognition program inside NDM will analyze the deformation, thus antibiotic situation in sample can be accurately told. Hope with our project, antibiotics issues can be solved in an accelerated way.
Contact us:
Weibo
ustc.igem.2015@gmail.com
NoteBook
Our notebook has three sections:
- Timeline:The record of our laboratory progress;
- Bibliography: The collection of literatures;
- Protocols:The experimental standards we applied.
We sincerely hope that you can gain something from our notes. If you have doubt of anything, please contact us!
Modeling
In our modeling efforts, we followed closely on our major 2 sub-projects, Color Imaging and Motion Control and their experimental connection. Conjugation Combing all the above, came the C. imager Modeling.
Human Practice
We strongly hold a belief that it is our arduous responsibility to not only develop synthetic biology by working on a project day and night but teach and enlighten the public to be intrigued in biology and understand what scientists are doing on genetic engineering and other biology technology so that the public is capable of judging whether genetically modified organism (GMO) is safe and personalized gene sequencing and therapy are reliable enough...