Team:Pitt/Estrogen/Project

Home

Project Overview Estrogen Sensor Protease Sensor Three-Hybrid Versatile Sensor Amplification and Quenching

Notebook Sensor Extract Protocol Daily Notebook

Parts

Human Practices

Team Meet us! Attributions

Sponsors

2015 iGEM Home

Estrogen Sensor

This project uses an estrogen-sensitive T7 RNA polymerase designed by the CMU iGEM team to activate transcription of a reporter gene in a cell-free extract. This system can be freeze-dried on paper for easy transportation, and is cheap to mass-produce. Due to the inherent amplification of transcription and translation, we hope to achieve limits of detection lower than current methods used.

As shown in the diagram above, in the presence of estrogen, the synthetic T7 RNA polymerase will initiate transcription of a reporter construct. The mRNA will then be translated into a reporter, which will either be easily visible on paper (e.g. a colored protein), or easily detectable by a fluorimeter (e.g. green fluorescent protein).