Team:Technion Israel/Lab Notebook/Overview
Lab Notebook Overview
Week 1: May 2-8
Bacillus
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E.coli
Planned the Gibson reaction for our gBlock of 3α-HSD and the plasmid N155 (from our mentors) containing pT7, RBS and mcherry.
Ordered the gBlock of 3α-HSD, which was optimized for E. coli codon usage. We also ordered primers for the Gibson reaction for the gBlock.
Performed reverse PCR to the N155 plasmid and carried out a DPN1 reaction on the product.
Active team members this week: Adi and Alexey
Modeling
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Comb
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NADPH
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Week 2: May 9-15
Bacillus
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E.coli
Our gBlock arrived!!!
We performed PCR on the 3αHSD gBlock with only 10 cycles (as recommended), but received no band in the gel
We performed PCR on the 3αHSD gBlock in higher temperatures, but still received no bands in the gel
Active team members this week: Adi
Modeling
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Comb
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NADPH
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Week 3: May 16-22
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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Week 4: May 23-29
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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Week 5: May 30-June 5
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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Week 6: June 6-12
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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Week 7: June 13-19
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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Week 8: June 20-26
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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Week 9: June 27-July 3
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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Week 10: July 4-10
Bacillus
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E.coli
We took a break for our exams.
Modeling
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Comb
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NADPH
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Week 11: July 11-17
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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Week 12: July 18-24
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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Week 13: July 25-31
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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Week 14: August 1-7
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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Week 15: August 8-14
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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Week 16: August 15-21
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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Week 17: August 22-28
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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Week 18: August 29-September 4
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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Week 19: September 5-11
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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Week 20: September 12-18
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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