Team:NTU-LIHPAO-Taiwan/Notebook

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July

7/4

Manufacture CP+ plate

7/6

Learn how to transform

Apoptosis part:

Transformation of:

[RBS] (BBa_B0034), [C2] (BBa_C0053)

7/7

Learn how to manufacture competent cell

Manufacture competent cell

PYY part:

Transformation of:

[promoter](BBa_J23100), [signal peptide](BBa_K554002), [terminator](BBa_B0015)

Apoptosis part:

Transformation of:

[pC1](BBa_R0051), [pC2](BBa_R0053), [endolysin](BBa_K112806), [C1](BBa_C0051)

7/7

Learn how to extract the plasmid

Learn how to conduct the gel extraction and electrophoresis

Apoptosis part:

Extract the plasmid:

[RBS](BBa_B0034), [C2](BBa_C0053)

Restriction enzyme:

[RBS] cleave by EcoRI, [C2] cleave by EcoRI and PstI

Gel electrophoresis:

[RBS] ----- Result: Failed (Smeared band was too blurred)

[C2] ----- Result: Succeeded

Transformation of:

[pC1](BBa_R0051),[pC2](BBa_R0053),[endolysin](BBa_K112806) again