Team:UiOslo Norway/Experiments/TOPO cloning

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TOPO Zero blunt cloning (Invitrogen):

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Use Zero blunt TOPO PCR cloning Kit. Link to the provided manual.

4 μl Purified PCR product
1 μl Salt Solution
1 μl Topo Vector (pCR4)

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6 μl Total Reaction

Incubate at Roomtemperature for 30 minutes