Team:Tuebingen/Description
Project Description
A Biosensor Memory Module: Cre Sensor
Analysis and imaging methods are an indispensable element of every scientific study - in a wider sense the results are only as good as their measurement methods.
Photostimulation is one of the most important non-invasive analysis methods allowing researchers to examine the relationship between metabolic processes, e.g. through activating a molecule via light treatment. We want to create a module which makes it possible to take a snapshot of the activity of a sensor at any time.
Our designed system should be capable of capturing such a ‘snapshot’ of the activity of a sensor. Therefore our aim is to create a Cre recombinase whose activity can be reversibly controlled by light. By activating this construct only for a short period of time, we can use a Cre to switch on expression of Luciferase in only a limited amount of the sensor cells. Through coupling the Cre expression to the sensor we can thereby permanently write the sensor state of a given time point into the DNA of a system.
We encourage you to put up much more information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page.
Part Table
<groupparts>iGEM015 Example</groupparts>
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