Team:UiOslo Norway/Experiments/Alkaline Phospatase

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Shrimp Alkaline Phosphatase Treatment (Fermentas):

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Following the provided protocol.

2 μl 10X Reaction Buffer
1 μl shrimp alkaline phosphatase
x μl DNA( 1 μg total concentration)
MilliQ up to 20 μl

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Total Reaction of 20 μl

Incubate at 37 °C for 30 minutes
Heat inactivation at 65 °C for 15 minutes.